BibTex format
@article{Higham:2023:10.1016/j.jinf.2023.02.035,
author = {Higham, SL and Baker, S and Flight, KE and Krishna, A and Kellam, P and Reece, ST and Tregoning, JS},
doi = {10.1016/j.jinf.2023.02.035},
journal = {Journal of Infection},
pages = {563--573},
title = {Intranasal immunisation with Outer Membrane Vesicles (OMV) protects against airway colonisation and systemic infection with Acinetobacter baumannii.},
url = {http://dx.doi.org/10.1016/j.jinf.2023.02.035},
volume = {86},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - OBJECTIVES: The multi-drug resistant bacteria Acinetobacter baumannii is a major cause of hospital associated infection; a vaccine could significantly reduce this burden. The aim was to develop a clinically relevant model of A. baumannii respiratory tract infection and to test the impact of different immunisation routes on protective immunity provided by an outer membrane vesicle (OMV) vaccine. METHODS: BALB/c mice were intranasally challenged with isolates of oxa23-positive global clone GC2 A. baumannii from the lungs of patients with ventilator associated pneumonia. Mice were immunised with OMVs by the intramuscular, subcutaneous or intranasal routes; protection was determined by measuring local and systemic bacterial load. RESULTS: Infection with A. baumannii clinical isolates led to a more disseminated infection than the prototype A. baumannii strain ATCC17978; with bacteria detectable in upper and lower airways and the spleen. Intramuscular immunisation induced an antibody response but did not protect against bacterial infection. However, intranasal immunisation significantly reduced airway colonisation and prevented systemic bacterial dissemination. CONCLUSION: Use of clinically relevant isolates of A. baumannii provides stringent model for vaccine development. Intranasal immunisation with OMVs was an effective route for providing protection, demonstrating that local immunity is important in preventing A. baumannii infection.
AU - Higham,SL
AU - Baker,S
AU - Flight,KE
AU - Krishna,A
AU - Kellam,P
AU - Reece,ST
AU - Tregoning,JS
DO - 10.1016/j.jinf.2023.02.035
EP - 573
PY - 2023///
SN - 0163-4453
SP - 563
TI - Intranasal immunisation with Outer Membrane Vesicles (OMV) protects against airway colonisation and systemic infection with Acinetobacter baumannii.
T2 - Journal of Infection
UR - http://dx.doi.org/10.1016/j.jinf.2023.02.035
UR - https://www.ncbi.nlm.nih.gov/pubmed/36858180
UR - https://www.sciencedirect.com/science/article/pii/S0163445323001226
VL - 86
ER -