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@article{Zafar:2024:10.1039/d4cb00093e,
author = {Zafar, A and Sridhar, S and Bineva-Todd, G and Cioce, A and Abdulla, N and Chang, V and Malaker, SA and Hewings, DS and Schumann, B},
doi = {10.1039/d4cb00093e},
journal = {RSC Chemical Biology},
pages = {1002--1009},
title = {Expanding the repertoire of GalNAc analogues for cell-specific bioorthogonal tagging of glycoproteins},
url = {http://dx.doi.org/10.1039/d4cb00093e},
volume = {5},
year = {2024}
}
TY - JOUR
AB - Glycosylation is a ubiquitous modification of proteins, necessitating approaches for its visualization and characterization. Bioorthogonally tagged monosaccharides have been instrumental to this end, offering a chemical view into the cell biology of glycans. Understanding the use of such monosaccharides by cellular biosynthetic pathways has expanded their applicability in cell biology, for instance through the strategy named Bio-Orthogonal Cell-specific TAgging of Glycoproteins (BOCTAG). Here, we show that the cellular use of two azide-tagged analogues of the monosaccharide N-acetylgalactosamine (GalNAzMe and GalNPrAz) can be promoted through expression of two biosynthetic enzymes. More precisely, cellular expression of the bacterial kinase NahK and the engineered human pyrophosphorylase AGX1F383A led to biosynthesis of the corresponding activated nucleotide-sugars and subsequent bioorthogonal tagging of the cellular glycoproteome. We explore the use of both sugars for BOCTAG, demonstrating the visualization of cell surface glycosylation tagged with GalNPrAz in a specific cell line in a co-culture system. Our work adds to the toolbox of glycoprotein analysis in biomedicine.
AU - Zafar,A
AU - Sridhar,S
AU - Bineva-Todd,G
AU - Cioce,A
AU - Abdulla,N
AU - Chang,V
AU - Malaker,SA
AU - Hewings,DS
AU - Schumann,B
DO - 10.1039/d4cb00093e
EP - 1009
PY - 2024///
SN - 2633-0679
SP - 1002
TI - Expanding the repertoire of GalNAc analogues for cell-specific bioorthogonal tagging of glycoproteins
T2 - RSC Chemical Biology
UR - http://dx.doi.org/10.1039/d4cb00093e
UR - https://www.ncbi.nlm.nih.gov/pubmed/39238612
UR - https://pubs.rsc.org/en/content/articlelanding/2024/cb/d4cb00093e
VL - 5
ER -