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BibTex format

@article{Cavuto:2025:10.1038/s41467-025-57647-3,
author = {Cavuto, ML and Malpartida, Cardenas K and Pennisi, I and Pond, M and Mirza, S and Moser, N and Comer, M and Stokes, I and Eke, L and Lant, S and Szostak, K and Miglietta, L and Stringer, O and Mantikas, K-T and Sumner, R and Bolt, F and Sriskandan, S and Holmes, A and Georgiou, P and Ulaeto, D and Maluquer, de Motes C and Rodriguez, Manzano J},
doi = {10.1038/s41467-025-57647-3},
journal = {Nature Communications},
title = {Portable molecular diagnostic platform forrapid point-of-care detection of mpox andother diseases},
url = {http://dx.doi.org/10.1038/s41467-025-57647-3},
volume = {16},
year = {2025}
}
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TY  - JOUR
AB  - The World Health Organization’s designation of mpox as a public health emergency of international concern in August 2024 underscores the urgent need for effective diagnostic solutions to combat this escalating threat. The rapid global spread of clade II mpox, coupled with the sustained human-to-human transmission of the more virulent clade I mpox in the Democratic Republic of Congo, highlights a critical gap in point-of-care diagnostics for this emergent disease. In response, we developed Dragonfly, a portable molecular diagnostic platform for point-of-care use that integrates power-free nucleic acid extraction (<5 minutes) with lyophilised colourimetric LAMP chemistry. The platform demonstrated an analytical limit-of-detection of 100 genome copies per reaction for monkeypox virus, effectively distinguishing it from other orthopoxviruses, herpes simplex virus, and varicella-zoster virus. Clinical validation on 164 samples, including 51 mpox-positive cases, yielded 96.1% sensitivity and 100% specificity for orthopoxviruses, and 94.1% sensitivity and 100% specificity for monkeypox virus. Here, we present a rapid, accessible, and robust point-of-care diagnostic solution for mpox, suitable for both low- and high-resource settings, addressing the global resurgence of orthopoxviruses in the context of declining smallpox immunity.
AU  - Cavuto,ML
AU  - Malpartida,Cardenas K
AU  - Pennisi,I
AU  - Pond,M
AU  - Mirza,S
AU  - Moser,N
AU  - Comer,M
AU  - Stokes,I
AU  - Eke,L
AU  - Lant,S
AU  - Szostak,K
AU  - Miglietta,L
AU  - Stringer,O
AU  - Mantikas,K-T
AU  - Sumner,R
AU  - Bolt,F
AU  - Sriskandan,S
AU  - Holmes,A
AU  - Georgiou,P
AU  - Ulaeto,D
AU  - Maluquer,de Motes C
AU  - Rodriguez,Manzano J
DO  - 10.1038/s41467-025-57647-3
PY  - 2025///
SN  - 2041-1723
TI  - Portable molecular diagnostic platform forrapid point-of-care detection of mpox andother diseases
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-025-57647-3
UR  - https://www.nature.com/articles/s41467-025-57647-3
VL  - 16
ER  -
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