In this section
@article{Gadola:2003:10.1098/rstb.2003.1267,
author = {Gadola, SD and Karadimitris, A and Zaccai, NR and Salio, M and Dulphy, N and Shepherd, D and Jones, EY and Cerundolo, V},
doi = {10.1098/rstb.2003.1267},
journal = {Philos Trans R Soc Lond B Biol Sci},
pages = {875--877},
title = {Generation of CD1 tetramers as a tool to monitor glycolipid-specific T cells.},
url = {http://dx.doi.org/10.1098/rstb.2003.1267},
volume = {358},
year = {2003}
}
TY - JOUR
AB - CD1 molecules are beta(2)m-associated HLA class-I-like glycoproteins which have the unique ability to present glycolipid and phospholipid antigens to specific T lymphocytes. To study the biology of CD1 and its role in human disease we developed novel techniques for generation of recombinant CD1/lipid complexes by in vitro refolding. Fluorescent tetrameric complexes made from soluble recombinant CD1d/alpha-galactosylceramide complexes allowed highly sensitive and specific ex vivo and in vitro detection and functional characterization of novel human T-lymphocyte populations. Furthermore, protein crystals were obtained from soluble recombinant CD1b/beta(2)m-proteins loaded either with phosphatidylinositol or ganglioside GM2, which led to the first atomic structure determination of a CD1/lipid complex. The analysis of these crystal structures clarified how CD1b molecules can bind lipid ligands of different size, and revealed a broader spectrum of potential CD1b ligands than previously predicted.
AU - Gadola,SD
AU - Karadimitris,A
AU - Zaccai,NR
AU - Salio,M
AU - Dulphy,N
AU - Shepherd,D
AU - Jones,EY
AU - Cerundolo,V
DO - 10.1098/rstb.2003.1267
EP - 877
PY - 2003///
SN - 0962-8436
SP - 875
TI - Generation of CD1 tetramers as a tool to monitor glycolipid-specific T cells.
T2 - Philos Trans R Soc Lond B Biol Sci
UR - http://dx.doi.org/10.1098/rstb.2003.1267
UR - https://www.ncbi.nlm.nih.gov/pubmed/12803920
VL - 358
ER -