In this section
@article{Roberts:2009,
author = {Roberts, AP and Abaitua, F and O'Hare, P and McNab, D and Rixon, FJ and Pasdeloup, D},
journal = {J. Virol.},
pages = {105--116},
title = {Differing Roles of Inner Tegument Proteins pUL36 and pUL37 During Entry of Herpes Simplex Virus Type 1 (HSV-1)},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18971278},
volume = {83},
year = {2009}
}
TY - JOUR
AB - Studies with Herpes simplex virus type 1 (HSV-1) have shown that secondary envelopment and virus release are blocked in mutants deleted for the tegument protein genes UL36 or UL37, leading to the accumulation of DNA-containing capsids in the cytoplasm of infected cells. The failure to assemble infectious virions has meant that the role of these genes in initial stages of infection could not be investigated. To circumvent this, cells infected at low multiplicity were fused to form syncytia, thereby allowing capsids released from infected nuclei access to uninfected nuclei without having to cross a plasma membrane. Visualisation of virus DNA replication showed that a UL37-minus mutant was capable of transmitting infection to all the nuclei within a syncytium as efficiently as HSV-1 WT, whereas infection by UL36-minus mutants failed to spread. Thus, these inner tegument proteins have differing functions, with pUL36 being essential during both the assembly and uptake stages of infection, whilst pUL37 is needed for formation of virions but is not required during the initial stages of infection. Analysis of non-infectious enveloped particles (L-particles) further showed that pUL36 and pUL37 are dependent on each other for incorporation into tegument.
AU - Roberts,AP
AU - Abaitua,F
AU - O'Hare,P
AU - McNab,D
AU - Rixon,FJ
AU - Pasdeloup,D
EP - 116
PY - 2009///
SP - 105
TI - Differing Roles of Inner Tegument Proteins pUL36 and pUL37 During Entry of Herpes Simplex Virus Type 1 (HSV-1)
T2 - J. Virol.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18971278
VL - 83
ER -