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BibTex format

@article{Fan:2009:10.1371/journal.ppat.1000409,
author = {Fan, S and Gao, Y and Shinya, K and Li, CK and Li, Y and Shi, J and Jiang, Y and Suo, Y and Tong, T and Zhong, G and Song, J and Zhang, Y and Tian, G and Guan, Y and Xu, X-N and Bu, Z and Kawaoka, Y and Chen, H},
doi = {10.1371/journal.ppat.1000409},
journal = {PLoS Pathogens},
title = {Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates},
url = {http://dx.doi.org/10.1371/journal.ppat.1000409},
volume = {5},
year = {2009}
}
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TY  - JOUR
AB  - The continued spread of highly pathogenic H5N1 influenza viruses among poultry and wild birds, together with the emergence of drug-resistant variants and the possibility of human-to-human transmission, has spurred attempts to develop an effective vaccine. Inactivated subvirion or whole-virion H5N1 vaccines have shown promising immunogenicity in clinical trials, but their ability to elicit protective immunity in unprimed human populations remains unknown. A cold-adapted, live attenuated vaccine with the hemagglutinin (HA) and neuraminidase (NA) genes of an H5N1 virus A/VN/1203/2004 (clade 1) was protective against the pulmonary replication of homologous and heterologous wild-type H5N1 viruses in mice and ferrets. In this study, we used reverse genetics to produce a cold-adapted, live attenuated H5N1 vaccine (AH/AAca) that contains HA and NA genes from a recent H5N1 isolate, A/Anhui/2/05 virus (AH/05) (clade 2.3), and the backbone of the cold-adapted influenza H2N2 A/AnnArbor/6/60 virus (AAca). AH/AAca was attenuated in chickens, mice, and monkeys, and it induced robust neutralizing antibody responses as well as HA-specific CD4+ T cell immune responses in rhesus macaques immunized twice intranasally. Importantly, the vaccinated macaques were fully protected from challenge with either the homologous AH/05 virus or a heterologous H5N1 virus, A/bar-headed goose/Qinghai/3/05 (BHG/05; clade 2.2). These results demonstrate for the first time that a cold-adapted H5N1 vaccine can elicit protective immunity against highly pathogenic H5N1 virus infection in a nonhuman primate model and provide a compelling argument for further testing of double immunization with live attenuated H5N1 vaccines in human trials.
AU  - Fan,S
AU  - Gao,Y
AU  - Shinya,K
AU  - Li,CK
AU  - Li,Y
AU  - Shi,J
AU  - Jiang,Y
AU  - Suo,Y
AU  - Tong,T
AU  - Zhong,G
AU  - Song,J
AU  - Zhang,Y
AU  - Tian,G
AU  - Guan,Y
AU  - Xu,X-N
AU  - Bu,Z
AU  - Kawaoka,Y
AU  - Chen,H
DO  - 10.1371/journal.ppat.1000409
PY  - 2009///
SN  - 1553-7366
TI  - Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates
T2  - PLoS Pathogens
UR  - http://dx.doi.org/10.1371/journal.ppat.1000409
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000267085800053&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL  - 5
ER  -
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