In this section
@article{Kim:2014:ndt/gfu019,
author = {Kim, MJ and Turner, CM and Hewitt, R and Smith, J and Bhangal, G and Pusey, CD and Unwin, RJ and Tam, FWK},
doi = {ndt/gfu019},
journal = {Nephrology Dialysis Transplantation},
pages = {1350--1361},
title = {Exaggerated renal fibrosis in P2X4 receptor-deficient mice following unilateral ureteric obstruction},
url = {http://dx.doi.org/10.1093/ndt/gfu019},
volume = {29},
year = {2014}
}
TY - JOUR
AB - Background. The ATP-sensitive P2X7 receptor (P2X7R) hasbeen shown to contribute to renal injury in nephrotoxic nephritis,a rodent model of acute glomerulonephritis, and in unilateral uretericobstruction (UUO), a rodent model of chronic interstitialinflammation and fibrosis. Renal tubular cells, endothelial cellsand macrophages also express the closely related P2X4 receptor(P2X4R), which is chromosomally co-located with P2X7R andhas 40% homology; it is also pro-inflammatory and has beenshown to interact with P2X7R to modulate its pro-apoptotic andpro-inflammatory effects. Therefore, we chose to explore the functionof P2X4R in the UUO model of renal injury using knockoutmice. We hypothesized that UUO-induced tubulointerstitialdamage and fibrosis would also be attenuated in P2X4R−/− mice.Method. P2X4R−/− and wild-type (WT) mice were subjectedto either UUO or sham operation. Kidney samples taken onDays 7 and 14 were evaluated for renal inflammation and fi-brosis, and expression of pro-fibrotic factors.Results. To our surprise, the obstructed kidney in P2X4R−/−mice showed more severe renal injury, more collagen deposition( picrosirius red staining, increase of 53%; P < 0.05) andmore type I collagen staining (increase of 107%; P < 0.01), aswell as increased mRNA for TGF-β (increase of 102%, P <0.0005) and CTGF (increase of 157%; P < 0.05) by Day 14,compared with the UUO WT mice.Conclusion. These findings showed that lack of P2X4Rexpression leads to increased renal fibrosis, and increasedexpression of TGF-β and CTGF in the UUO model.
AU - Kim,MJ
AU - Turner,CM
AU - Hewitt,R
AU - Smith,J
AU - Bhangal,G
AU - Pusey,CD
AU - Unwin,RJ
AU - Tam,FWK
DO - ndt/gfu019
EP - 1361
PY - 2014///
SN - 1460-2385
SP - 1350
TI - Exaggerated renal fibrosis in P2X4 receptor-deficient mice following unilateral ureteric obstruction
T2 - Nephrology Dialysis Transplantation
UR - http://dx.doi.org/10.1093/ndt/gfu019
VL - 29
ER -