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@article{Majoor:2014:10.1186/1465-9921-15-14,
author = {Majoor, CJ and de, Pol MAV and Kamphuisen, PW and Meijers, JCM and Molenkamp, R and Wolthers, KC and van, der Poll T and Nieuwland, R and Johnston, SL and Sterk, PJ and Bel, EHD and Lutter, R and van, der Sluijs KF},
doi = {10.1186/1465-9921-15-14},
journal = {Respiratory Research},
title = {Evaluation of coagulation activation after Rhinovirus infection in patients with asthma and healthy control subjects: an observational study},
url = {http://dx.doi.org/10.1186/1465-9921-15-14},
volume = {15},
year = {2014}
}
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TY  - JOUR
AB  - BackgroundAsthma exacerbations are frequently triggered by rhinovirus infections. Both asthma and respiratory tract infection can activate haemostasis. Therefore we hypothesized that experimental rhinovirus-16 infection and asthmatic airway inflammation act in synergy on the haemostatic balance.Methods28 patients (14 patients with mild allergic asthma and 14 healthy non-allergic controls) were infected with low-dose rhinovirus type 16. Venous plasma and bronchoalveolar lavage fluid (BAL fluid) were obtained before and 6 days after infection to evaluate markers of coagulation activation, thrombin-antithrombin complexes, von Willebrand factor, plasmin-antiplasmin complexes, plasminogen activator inhibitor type-1, endogenous thrombin potential and tissue factor-exposing microparticles by fibrin generation test, in plasma and/or BAL fluid. Data were analysed by nonparametric tests (Wilcoxon, Mann Whitney and Spearman correlation).Results13 patients with mild asthma (6 females, 19-29 y) and 11 healthy controls (10 females, 19-31 y) had a documented Rhinovirus-16 infection. Rhinovirus-16 challenge resulted in a shortening of the fibrin generation test in BAL fluid of asthma patients (t = -1: 706 s vs. t = 6: 498 s; p = 0.02), but not of controls (t = -1: 693 s vs. t = 6: 636 s; p = 0.65). The fold change in tissue factor-exposing microparticles in BAL fluid inversely correlated with the fold changes in eosinophil cationic protein and myeloperoxidase in BAL fluid after virus infection (r = -0.517 and -0.528 resp., both p = 0.01).Rhinovirus-16 challenge led to increased plasminogen activator inhibitor type-1 levels in plasma in patients with asthma (26.0 ng/mL vs. 11.5 ng/mL in healthy controls, p = 0.04). Rhinovirus-16 load in BAL showed a linear correlation with the fold change in endogenous thrombin potential, plasmin-antiplasmin complexes and pla
AU  - Majoor,CJ
AU  - de,Pol MAV
AU  - Kamphuisen,PW
AU  - Meijers,JCM
AU  - Molenkamp,R
AU  - Wolthers,KC
AU  - van,der Poll T
AU  - Nieuwland,R
AU  - Johnston,SL
AU  - Sterk,PJ
AU  - Bel,EHD
AU  - Lutter,R
AU  - van,der Sluijs KF
DO  - 10.1186/1465-9921-15-14
PY  - 2014///
SN  - 1465-993X
TI  - Evaluation of coagulation activation after Rhinovirus infection in patients with asthma and healthy control subjects: an observational study
T2  - Respiratory Research
UR  - http://dx.doi.org/10.1186/1465-9921-15-14
VL  - 15
ER  -
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