BibTex format
@article{Lowe:2015:10.1513/annalsats.201507-463oc,
author = {Lowe, DM and Bangani, N and Goliath, R and Kampmann, B and Wilkinson, KA and Wilkinson, RJ and Martineau, AR},
doi = {10.1513/annalsats.201507-463oc},
journal = {Annals of the American Thoracic Society},
pages = {1627--1637},
title = {Effect of Antiretroviral Therapy on HIV-mediated Impairment of the Neutrophil Antimycobacterial Response.},
url = {http://dx.doi.org/10.1513/annalsats.201507-463oc},
volume = {12},
year = {2015}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - <h4>Rationale</h4>Experimental and epidemiological evidence suggests that neutrophils are important in the host response to tuberculosis. HIV infection, which increases the risk of tuberculosis, adversely affects neutrophil function.<h4>Objectives</h4>To determine the impact of HIV and antiretroviral therapy on neutrophil antimycobacterial activity.<h4>Methods</h4>We performed a cross-sectional comparison of neutrophil functions in 20 antiretroviral-naive HIV-infected and 20 HIV-uninfected individuals using luminescence-, flow cytometry-, and ELISA-based assays. We then conducted a prospective study in the HIV-infected individuals investigating these parameters during the first 6 months of antiretroviral therapy. Surface markers of neutrophil activation were investigated in a separate cohort using flow cytometry.<h4>Measurements and main results</h4>HIV infection impaired control of Mycobacterium tuberculosis by neutrophils (mean ratio of mycobacterial luminescence in neutrophil samples vs. serum controls at 1 hour in HIV-infected participants, 0.88 ± 0.13 vs. HIV-uninfected participants, 0.76 ± 0.14; P = 0.01; at 24 hours, 0.82 ± 0.13 vs. 0.71 ± 0.13; P = 0.01). The extent of impairment correlated with log[HIV viral load]. Neutrophil cell death after 24 hours' incubation with M. tuberculosis was higher in the HIV-infected cohort (85.3 ± 11.8% vs. 57.9 ± 22.4% necrotic cells; P < 0.0001). Neutrophils from HIV-infected participants demonstrated significantly more CD62L-negative cells (median, 23.0 vs. 8.5%; P = 0.008) and CD16-negative cells (3.2 vs. 1.3%, P = 0.03). Antiretroviral therapy restored mycobacterial restriction and pattern of neutrophil death toward levels seen in HIV-uninfected persons.<h4>Conclusions<
AU - Lowe,DM
AU - Bangani,N
AU - Goliath,R
AU - Kampmann,B
AU - Wilkinson,KA
AU - Wilkinson,RJ
AU - Martineau,AR
DO - 10.1513/annalsats.201507-463oc
EP - 1637
PY - 2015///
SN - 2329-6933
SP - 1627
TI - Effect of Antiretroviral Therapy on HIV-mediated Impairment of the Neutrophil Antimycobacterial Response.
T2 - Annals of the American Thoracic Society
UR - http://dx.doi.org/10.1513/annalsats.201507-463oc
VL - 12
ER -