In this section
@article{Keogh:1985,
author = {Keogh, JM and Matthews, PM and Seymour, AM and Radda, GK},
journal = {Adv Myocardiol},
pages = {299--309},
title = {A phosphorus-31 nuclear magnetic resonance study of effects of altered thyroid state on cardiac bioenergetics.},
url = {https://www.ncbi.nlm.nih.gov/pubmed/2986261},
volume = {6},
year = {1985}
}
TY - JOUR
AB - We have used 31P nuclear magnetic resonance (NMR) techniques to characterize bioenergetic changes in the Langendorff-perfused rat heart accompanying alterations in thyroid state. Cytosolic phosphocreatine and inorganic phosphate concentrations changed significantly in both the hypo- and hyperthyroid groups compared to controls; the calculated phosphorylation potential [( ATP]/[ADP][Pi]) increased by 60% in hypothyroidism and decreased by 60% in hyperthyroidism relative to the euthyroid value of 47 X 10(3) M-1. Creatine phosphokinase (CPK) and mitochondrial ATP synthase rates were measured in the intact tissue using a saturation-transfer NMR method. There were no significant differences in the measured fluxes through the CPK reaction among the three groups (4.24 +/- 1.00 mM X sec-1 for the euthyroid group). Although O2 consumption increased by 46% in hearts from hyperthyroid animals, no change in the measured mitochondrial ATP synthase flux was observed compared to the euthyroid flux of 1.05 +/- 0.11 mM X sec-1. These results suggest that the apparent in situ P/O ratio of mitochondria in hearts from hyperthyroid animals is reduced relative to that in euthyroid controls.
AU - Keogh,JM
AU - Matthews,PM
AU - Seymour,AM
AU - Radda,GK
EP - 309
PY - 1985///
SN - 0270-4056
SP - 299
TI - A phosphorus-31 nuclear magnetic resonance study of effects of altered thyroid state on cardiac bioenergetics.
T2 - Adv Myocardiol
UR - https://www.ncbi.nlm.nih.gov/pubmed/2986261
VL - 6
ER -