In this section
@article{Mason:2002:10.1152/ajpcell.00339.2001,
author = {Mason, JC and Lidington, EA and Ahmad, SR and Haskard, DO},
doi = {10.1152/ajpcell.00339.2001},
journal = {American Journal of Physiology Cell Physiology},
pages = {C578--C587},
title = {bFGF and VEGF synergistically enhance endothelial cytoprotection via decay-accelerating factor induction},
url = {http://dx.doi.org/10.1152/ajpcell.00339.2001},
volume = {282},
year = {2002}
}
TY - JOUR
AB - The complement-regulatory protein decay-accelerating factor (DAF) can be upregulated on endothelial cells (EC) by protein kinase C (PKC)-dependent and -independent pathways. We hypothesized that basic fibroblast growth factor (bFGF) might induce EC DAF expression, providing a cytoprotective mechanism for angiogenic neovessels against complement-mediated injury. Incubation of umbilical vein, aortic, and dermal EC with bFGF or vascular endothelial growth factor (VEGF) significantly increased DAF expression. Growth factor-induced EC proliferation was inhibited by PKC antagonists. In contrast, although PKC antagonists inhibited VEGF-induced DAF expression, bFGF-induced DAF was unaffected. Investigation of mitogen-activated kinase (MAPK) pathways also revealed differences, with bFGF-induced DAF dependent on p44/42 and p38 MAPK and VEGF requiring activation of p38 MAPK alone. Upregulation of DAF by bFGF was functionally relevant, reducing C3 deposition on EC after complement activation by 60% and resulting in marked reduction in complement-mediated EC lysis. bFGF and VEGF were synergistic in terms of DAF expression, resulting in enhanced cytoprotection. These observations reveal parallel PKC-dependent and -independent pathways regulating complement activation during angiogenesis. Further elucidation of these pathways may provide important insights into innate cytoprotective mechanisms in endothelium.
AU - Mason,JC
AU - Lidington,EA
AU - Ahmad,SR
AU - Haskard,DO
DO - 10.1152/ajpcell.00339.2001
EP - 587
PY - 2002///
SN - 0363-6143
SP - 578
TI - bFGF and VEGF synergistically enhance endothelial cytoprotection via decay-accelerating factor induction
T2 - American Journal of Physiology Cell Physiology
UR - http://dx.doi.org/10.1152/ajpcell.00339.2001
VL - 282
ER -