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  • Book chapter
    Omer OS, Powell N, Lord GM, 2020,

    Characterizing Innate Lymphoid Cell Phenotype and Function in Human Inflammatory Bowel Disease

    , INNATE LYMPHOID CELLS, Editors: Amarnath, Publisher: HUMANA PRESS INC, Pages: 199-211, ISBN: 978-1-0716-0337-6
  • Conference paper
    Garcia E, Guo W, Kumar S, Gorlitz F, Sparks H, Alexandrov Y, Munro I, Kelly DJ, Warren S, Chennell G, Sardini A, Carling D, Thorpe P, Dunsby C, French PMWet al., 2020,

    FLIM, FRET and high content analysis

    , Symposium on Multiphoton Microscopy in the Biomedical Sciences XX held at SPIE BiOS Conference, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
  • Conference paper
    Gong S, Bahri M, Bronstein MM, Zafeiriou Set al., 2020,

    Geometrically Principled Connections in Graph Neural Networks

    , IEEE/CVF Conference on Computer Vision and Pattern Recognition (CVPR), Publisher: IEEE COMPUTER SOC, Pages: 11412-11421, ISSN: 1063-6919
  • Journal article
    Kulon D, Guler RA, Kokkinos I, Bronstein MM, Zafeiriou Set al., 2020,

    Weakly-Supervised Mesh-Convolutional Hand Reconstruction in the Wild

    , 2020 IEEE/CVF CONFERENCE ON COMPUTER VISION AND PATTERN RECOGNITION (CVPR), Pages: 4989-4999, ISSN: 1063-6919
  • Journal article
    Limbu S, Higgins CA, 2020,

    Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion

    , MOLECULAR DERMATOLOGY, Vol: 2154, Pages: 91-103, ISSN: 1064-3745
  • Journal article
    Entwistle LJ, Puttur F, Gregory LG, Lloyd CMet al., 2020,

    Group 2 ILC Functional Assays in Allergic Airway Inflammation

    , INNATE LYMPHOID CELLS, Vol: 2121, Pages: 99-114, ISSN: 1064-3745
  • Conference paper
    Gorlitz F, Wysoczanski R, Kumar S, Lightley J, Garcia E, Alexandrov Y, Munro I, Johnson S, Kehoe M, Hollick C, Graham J, Donnelly L, Barnes P, Dunsby C, Neil MAA, French PMWet al., 2020,

    Towards easier, faster super-resolved microscopy

    , Conference on Single Molecule Spectroscopy and Superresolution Imaging XIII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X
  • Journal article
    Cocker ATH, Shah NM, Raj I, Dermont S, Khan W, Mandalia S, Imami N, Johnson MRet al., 2020,

    Pregnancy Gestation Impacts on HIV-1-Specific Granzyme B Response and Central Memory CD4 T Cells.

    , Front Immunol, Vol: 11

    Pregnancy induces alterations in peripheral T-cell populations with both changes in subset frequencies and anti-viral responses found to alter with gestation. In HIV-1 positive women anti-HIV-1 responses are associated with transmission risk, however detailed investigation into both HIV-1-specific memory responses associated with HIV-1 control and T-cell subset changes during pregnancy have not been undertaken. In this study we aimed to define pregnancy and gestation related changes to HIV-1-specific responses and T-cell phenotype in ART treated HIV-1 positive pregnant women. Eleven non-pregnant and 24 pregnant HIV-1 positive women were recruited, peripheral blood samples taken, fresh cells isolated, and compared using ELISpot assays and flow cytometry analysis. Clinical data were collected as part of standard care, and non-parametric statistics used. Alterations in induced IFNγ, IL-2, IL-10, and granzyme B secretion by peripheral blood mononuclear cells in response to HIV-1 Gag and Nef peptide pools and changes in T-cell subsets between pregnant and non-pregnant women were assessed, with data correlated with participant clinical parameters and longitudinal analysis performed. Cross-sectional comparison identified decreased IL-10 Nef response in HIV-1 positive pregnant women compared to non-pregnant, while correlations exhibited reversed Gag and Nef cytokine and protease response associations between groups. Longitudinal analysis of pregnant participants demonstrated transient increases in Gag granzyme B response and in the central memory CD4 T-cell subset frequency during their second trimester, with a decrease in CD4 effector memory T cells from their second to third trimester. Gag and Nef HIV-1-specific responses diverge with pregnancy time-point, coinciding with relevant T-cell phenotype, and gestation associated immunological adaptations. Decreased IL-10 Nef and both increased granzyme B Gag response and central memory CD4 T cells implies that amplified a

  • Journal article
    Graziani G, Herget G, Ihorst G, Zeissig M, Chaidos A, Auner H, Duyster J, Waesch R, Engelhardt Met al., 2020,

    Time from first symptom onset to the final diagnosis of Multiple Myeloma (MM) - possible risks and future solutions: retrospective and prospective ‘Deutsche Studiengruppe MM’ (DSMM) and ‘European Myeloma Network’ (EMN) analysis

    , Leukemia and Lymphoma, Vol: 61, Pages: 875-886, ISSN: 1026-8022

    Multiple Myeloma (MM) often presents with unspecific symptoms and is challenging to diagnose. We performed this DSMM/EMN-analysis via test-(retro-) and validation (prospective) study to determine the time interval from the onset of first symptoms to the diagnosis of MM. The retrospective and prospective analyses were performed in 101 and 176 patients, respectively. The median time from first symptoms to the MM diagnosis in both cohorts was 4 and 6 months, respectively. Frequencies of MM-related pathologic bone fractures, renal and infectious complications at diagnosis occurred in 41%, 35% and 16% of patients, respectively. Our MM-questionnaire determined that 39% of patients were dissatisfied with the diagnostic process. PFS and OS proved insignificantly different with shorter (≤6) and longer (>6 months) latency periods. In conclusion, our in depth studies demonstrate that delays in diagnosis do not decrease PFS or OS, but induce MM-related complications and influence patients' satisfaction with their medical care.

  • Book chapter
    Vilar R, 2020,

    Interaction of metal complexes with G-quadruplex DNA

    , MEDICINAL CHEMISTRY, Editors: Sadler, VanEldik, Publisher: ELSEVIER ACADEMIC PRESS INC, Pages: 425-445, ISBN: 978-0-12-819196-5

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