HCF4- NIKON AX R MP NSPARC Confocal

GENERAL SPECIFICATIONS

  • A powerful multiphoton, confocal, super-resolution, and single-photon detecting inverted imaging system from NIKON: AX R MP with NSPARC. It is based on the ECLIPSE Ti2-E main body and supports a maximum Field of View (FOV) of 25 mm, providing an expansive space under the objective to easily accommodate varied specimens and large sample sizes.
  • Scan Head allows users to capture "bigger pictures, fast" by collecting more data per single frame without the need to constantly change objectives. It supports line scanning, bi-direction scanning, and averaging, with a continuously variable zoom ranging from 1X to 1000X.
  • Standard Image Acquisition (Galvano): Engineered for high-definition deep imaging, the Galvano scanner features a pixel size of up to 8192 x 8192 pixels, enabling Nyquist sampling at any magnification to ensure large images are captured without sacrificing a single detail.
  • High-Speed Image Acquisition (Resonant): The resonant scanner captures the rapid dynamics of life with a 2K resolution capable of up to 2048 x 2048 pixels, ensuring fast, gentle, and high-resolution imaging that keeps delicate live samples healthier for longer periods.
  • Super-Resolution & Macro-to-Micro Imaging: The integration of the NSPARC detector allows the system to seamlessly support the entire range of imaging applications—from macro imaging via high-speed image stitching to super-resolution micro imaging—within a single experiment.
  • Live Cell & High-Throughput Imaging: The system provides full support for live tissue and live cell imaging, featuring comprehensive environmental control including temperature regulation and CO2 supply (premixed gas) via the integrated Bold Line cage incubator. Additionally, there is the option to perform complex high-throughput imaging of tissue and multi-well plates, fully supported by the system's motorized XY stages and dedicated multi-well plate holders.
  • Time-Resolved Expansion: The system is upgraded with a PicoQuant turn-key FLIM and FCS kit.

MICROSCOPE OBJECTIVES

ILLUMINATION

  • Spectra Physics Insight X3+ Dual MP Laser featuring a fixed 1045 nm output and a broad tuning range of 680 nm to 1300 nm, delivering >3.5 W of peak power.
  • Six-line Laser Unit combining 405, 445, 488, 514, 561, and 640nm lines.
  • Fluorescence LED Illumination system offering four individually controllable color channels at 385nm, 475nm, 550nm, and 621nm.

DETECTORS

  • AX R MP Scan Head capable of both point scanning for confocal and multiphoton modalities over a 22mm FOV. It incorporates galvano mirrors for high-resolution imaging up to 8192x8192 and resonant mirrors for high-throughput gentle live-cell imaging up to 2048x2048.
  • 4Ch Detector Unit equipped with two tunable detection paths and two filter wheel paths, covering a detection range from 400nm to 920nm.
  • Non-Descanned Detector (NDD) utilizing 4 GaAsP photomultiplier tubes (PMTs) to collect visible light in the EPI direction from IR-excited dyes without confocal sectioning. 
  • Diascopic Detector Unit for transmitted images and Second Harmonic Generation (SHG) signals utilizing an excitation laser up to IR.
  • NSPARC Unit utilizing Image Scanning Microscopy (ISM) to improve resolution up to 100nm laterally and 300nm axially using visible and IR lasers.
  • Picoquant 2 Detector FLIM for AX MP, offering comprehensive FLIM, FRET, and FCS capabilities with 2 detection channels, measuring fluorescence lifetimes from 100 ps to microseconds.
  • Orca-Flash4.0v3 and Basler MED acA2440-75uc Color cameras for standard imaging tasks.

SOFTWARE

  • NIS-Elements C-ER for fully automated camera and confocal system control, supporting 6D imaging, Z-stacks, spectral unmixing, stitching, multipoint experiments, and programmable macro generation.
  • NIS-A 2D/3D Deconvolution utilizing both Blind and Non-blind algorithms to improve image clarity.
  • NIS-Elements Module NIS.Ai featuring AI-driven workflows including Enhance.ai, Convert.ai, and Segment.ai. 
  • NIS-A FLIM Module and SymPhoTime 64 Software (NovaFLIM) for dedicated lifetime data acquisition and advanced analysis scripting.
  • NIS-A General Analysis (GA3) and NIS-A JOBS Editor for creating complex 3D analysis workflows and autonomous experiment design.

ADD-ONS

  • Piezo Stage offering closed-loop control and 500 microns of high-precision Z-axis nanopositioning.
  • Water Immersion Dispenser to supply pure water to the objective tip, facilitating long-term unassisted immersion observation.
  • Perfect Focus System (v4) with a motorized nosepiece featuring an altered notch filter specifically modified for multiphoton use.