HCF3 - Leica Stellaris STED FALCON
Leica STELLARIS Tau-STED FALCON FLIM/ FCCS/ FLCS
Advantages of this microscope
- Tau-STED 2D/3D + 3 Depletion Lasers - 592, 660, 775nm
- STED White Objectives - 100x 1.40NA Oil + 86x 1.20NA Water (Motorised Correction)
- FALCON FLIM + FCCS + FLCS
- Phasor Analysis
- Confocal Super Resolution LASX Lightning Expert
- DMi8 CS Inverted Motorised Microscope
- Incubation Chamber - Cage Type
- Stage Top Incubator (Z-Galvanometer), CO2 Mixer and Humidity
- BF Detector for DMi 8
- LED Epiflfluorescence
- XY Scanning Stage + Z Galvanometer Stage - LASX Navigator Software
- White Laser 440-790nm + Pulse Picker
- 405nm Diode
- 8 kHz Tandem Scanner + FOV Scanner - Software Switching
- 4 Channel Spectral Dectection - 2 HyDS + HyDX + HyDR
Hammersmith Campus, ICTEM (L-Block) room 312
SN:8300000358
NB All users must have completed the on-line “Introduction to Laser Safety” from Imperial College safety department
Quickstart guides
Quickstart guide - Confocal HCF3 Leica Stellaris 8 Inverted (PDF)
Quickstart guide - STED HCF3 Leica Stellaris 8 Inverted (PDF)
Tiling and Multi-position Imaging (PDF)
STED
Leica Tutorial - Tau-STED. Explains the theory and also how to perform the Tau-STED experiments within the software:
STED Sample Preparation (PDF)
FALCON
Phasor-FLIM (YouTube) Phasor-FLIM (highly recommend, a very good seminar from the original group (Enrico Gratton) FRET trajectory is also explained.
Leica Tutorials - FALCON Phasor analysis. In the tutorial, many aspects of the software are covered and a tutorial on how to calculate FRET using Phasors on Alexa488 Tubulin and Alexa 555 Tubulin example data and removing unwanted signals using the phasor approach
Application Note: SP8 FALCON: a novel concept in fluorescence lifetime imaging enabling video-rate confocal FLIM
Leica Objectives
Offline software