HSR1 - ZEISS Lattice SIM 5

We are pleased to announce a new super-resolution structured illumination microscope will be coming to ICTEM in November 2026. We will be introducing a ZEISS Lattice SIM 5 super-resolution microscope on the Axio Observer 7 SR inverted stand to expand our super-resolution portfolio.
Lattice structured illumination achieves 60 nm lateral resolution for fast, gentle, multi-colour 3D super-resolution imaging of fixed and live specimens.
General specifications
  • Axio Observer 7 SR RP – Inverted Research Microscope for Lattice SIM
  • Definite Focus 3 hardware autofocus
  • Motorised XY scanning stage: 130 × 100 mm travel, 0.1 µm resolution
  • Z PIEZO WSB 500: 500 µm travel, 1 nm resolution
  • AI Sample Finder with overview camera for rapid sample navigation
  • Stage-top incubation: temperature and CO₂ control
  • Automated water immersion module with pipette
  • Slide, dish, chamber slide and multiwell formats
Imaging modalities
The system operates in two structured illumination modes:
  • Lattice SIM (super-resolution) – lattice-patterned illumination with linear and iterative reconstruction achieving 60 nm lateral resolution. Requires high-NA objectives (NA ≥ 1.2). Suitable for detailed structural imaging of fixed and live samples at sub-diffraction resolution in up to four colours.
  • SIM Apotome (optical sectioning) – grid-projection structured illumination for optical sectioning at diffraction-limited resolution. Compatible with all objectives on the system, including lower-magnification air objectives. Provides confocal-like sectioning from widefield images with high speed and low phototoxicity.
Microscope objectives
★ Suitable for Lattice SIM super-resolution (NA ≥ 1.2). All eight objectives can be used for SIM Apotome, widefield and transmitted-light imaging.
 
Illumination
  • Lattice SIM illumination module with 4 laser lines: All directly modulated, 500:1 dynamic range
    • 405 nm – 50 mW (diode)
    • 488 nm – 50 mW (diode)
    • 561 nm – 50 mW (diode, SHG)
    • 640 nm – 50 mW (diode)
  • Widefield: Viluma 9 Type XT 920Z LED with filter sets 38 HE (GFP), 96 HE (BFP), 90 HE (DAPI/FITC/TRITC/Cy5), 91 HE (CFP/YFP/mCherry), 112 HE (DAPI/FITC/DsRed/Cy5/Cy7)
  • Transmitted light: VIS-LED 2 (430–720 nm) with LD 0.55 motorised condenser
Detectors
  • 2 × Hamamatsu ORCA Fusion BT (sCMOS, back-thinned; QE up to 95%; 6.5 µm pixel; 2304 × 2304; liquid-cooled)
  • DuoLink SR dual-camera adapter with QUAD filter module for simultaneous two-channel or fast-sequential four-channel acquisition
  • Filter set FLEX for Lattice SIM: 4 adjusted filter cubes (MBS 405/488/561/642) with dual and triple emission bands 
Software
  • ZEN 3.13 – acquisition, processing and analysis (CZI format)
  • ZEN Module Lattice SIM – linear and iterative SIM reconstruction
  • ZEN Toolkit Deconvolution – iterative 3D deconvolution (multi-GPU)
  • ZEN Toolkit Smart Acquisition – Experiment Designer, Guided Acquisition, Experiment Feedback
  • ZEN Toolkit Advanced Acquisition – Z-stacks, tiles & positions, software autofocus, EDF
  • ZEN Toolkit Developer – Python scripting IDE, TCP/IP and COM remote control
  • ZEN Toolkit 2D / 3D – Image Analysis Wizard, AI-ready segmentation, 3D viewer
  • arivis Pro ACA – large-scale 3D visualisation and analysis with Tracking, Neuron Tracing and AI modules
  • Dedicated processing/storage PC (81 TB RAID-5, RTX A5000) with full offline ZEN desk and Arivis Pro suite
  • ZEN and Arivis licences on a third offline processing PC

We will open the waiting list for trainings closer to the time. For any enquiries please contact us.

Users in current need for super-resolution are encouraged to use our Nikon NSPARC or one of our Leica STED systems