Scientists reveal how a type of white blood cells, called Natural Killer cells, protect the body from tumours and virus-infected cells - News release
Imperial College London News Release
Under STRICT EMBARGO until
22.00 London time / 17.00 US Eastern time
Tuesday 13 September 2011
Scientists reveal in more detail than ever before how white blood cells kill diseased tissue using deadly granules, in research published today in PLoS Biology.
- PLoS Biology
- University of Oxford
- Biolotechnology and Biological Sciences Research Council (BBSRC)
- Medical Research Council (MRC)
- Imperial's Facility for Imaging by Light Microscopy (FILM)
Related news stories:
The researchers, from Imperial College London and the University of Oxford, used 'optical' laser tweezers and a super-resolution microscope to see the inner workings of white blood cells at the highest resolution ever. The researchers describe how a white blood cell rearranges its scaffolding of actin proteins on the inside of its membrane, to create a hole through which it delivers deadly enzyme-filled granules to kill diseased tissue.
The study looked at a type of white blood cell called a Natural Killer (NK) cell that protects the body by identifying and killing diseased tissue.
"NK cells are important in our immune response to viruses and rogue tissues like tumours. They may also play a role in the outcome of bone marrow transplants by determining whether a recipient's body rejects or accepts the donated tissue," said Professor Daniel Davis, from the Department of Life Sciences at Imperial College London, who led the research.
The scientists hope that learning more about how NK cells identify which tissues to kill and initiate the killing process could lead to better healthcare for some patients. Professor Davis said: "In the future, drugs that influence where and when NK cells kill could be included in medical treatments, such as the targeted killing of tumours. They may also prove useful in preventing the unwanted destruction by NK cells that may occur in transplant rejection or some auto-immune diseases."
The new visual resolution of NK cell action is a result of a novel imaging technique developed in collaboration with physicists at Imperial, and the use of a super high-resolution microscope at the University of Oxford. The researchers immobilised an NK cell and its target using a pair of 'optical' laser tweezers so that the microscope could capture all the action at the interface between the cells. They then watched inside the NK cell as the actin filaments parted to create a tiny portal and the enzyme-filled granules moved to the portal, ready to pass out of the NK cell and onto the target to kill it.
Dr Alice Brown, also from the Department of Life Sciences at Imperial College London, and one of the researchers who carried out many of the experiments, said: "These previously undetectable events inside cells have never been seen in such high resolution. It is truly exciting to observe what happens when an NK cell springs into action."
The contact between an NK cell and its target is only about a hundredth of a millimetre across and the miniscule actin proteins and granules change position continuously over the few minutes from initial contact until the target is killed. The microscope has to be able to capture images quickly enough and in high enough visual detail in order to reveal their activity.
Most microscopes view images in the horizontal plane, so to view an interface between two cells at any other orientation would require 'stacks' of multiple horizontal images combined to make a 3D image. This significantly limits the speed at which cell dynamics can be viewed and reduces image quality.
Professor Paul French from the Department of Physics at Imperial College London, who helped develop the microscopy with colleagues in the Photonics Group, said: "Using laser tweezers to manipulate the interface between live cells into a horizontal orienta tion means our microscope can take many images of the cell contact interface in rapid succession. This has provided an unprecedented means to directly see dynamic molecular processes that go on between live cells."
Professor Ilan Davis, a Wellcome Trust Senior Fellow at the University of Oxford, whose group applies super resolution technique to basic cell biology research said: "Our microscope has given us unprecedented views inside living NK cells capturing a super-resolution 3D image of the cell structures at twice the normal resolution of conventional light microscope. This method, developed at University of California San Francisco by Professor John Sedat, maximizes the amount of light captured from the specimen while minimizing the amount of stray light inside the instrument."
This study was funded by the Medical Research Council (MRC), the Biotechnology and Biological Sciences Research Council (BBSRC) and a Marie Curie Intra-European Fellowship. It also benefited from a £150,000 award from the Rector's Research Excellence Prize to reward high academic achievement in blue skies research with significant potential. Professors Daniel Davis and Paul French hold Wolfson Royal Society Research Merit Awards.
- ENDS -
For further information please contact:
Research Media Officer
Imperial College London
Tel: +44(0)20 7594 6702
Out of hours duty press officer: +44(0)7803 886 248
Notes to editors:
1. Journal reference: Brown ACN, Oddos S, Dobbie IM, Alakoskela J-M, Parton RM, et al. (2011) "Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-Resolution Microscopy" PLoS Biol 9(9): e1001152. doi:10.1371/journal.pbio.1001152 is published in Public Library of Science PLoS Biology on 13 September 2011 and under STRICT EMBARGO until 22.00 London time / 17.00 US Eastern time Tuesday 13 September 2011.
A final version of the study can be obtained from: http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001152
2. Video download
High Resolution Live cell imaging showing an NK cell contacting a diseased cell. NK cell proteins essential to detecting the diseased cell organise into a ring within which ‘killing’ granules are delivered to the diseased cell. Narration by Dr Alice Brown. Film compiled by Warwick Bromley. Copyright Imperial College London.
3. About Imperial College London
Consistently rated amongst the world's best universities, Imperial College London is a science-based institution with a reputation for excellence in teaching and research that attracts 14,000 students and 6,000 staff of the highest international quality. Innovative research at the College explores the interface between science, medicine, engineering and business, delivering practical solutions that improve quality of life and the environment - underpinned by a dynamic enterprise culture.
Since its foundation in 1907, Imperial's contributions to society have included the discovery of penicillin, the development of holography and the foundations of fibre optics. This commitment to the application of research for the benefit of all continues today, with current focuses including interdisciplinary collaborations to improve global health, tackle climate change, develop sustainable sources of energy and address security challenges.
In 2007, Imperial College London and Imperia l College Healthcare NHS Trust formed the UK's first Academic Health Science Centre. This unique partnership aims to improve the quality of life of patients and populations by taking new discoveries and translating them into new therapies as quickly as possible.
4. About BBSRC
BBSRC invests in world-cla ss bioscience research and training on behalf of the UK public. Our aim is to further scientific knowledge to promote economic growth, wealth and job creation and to improve quality of life in the UK and beyond.
Funded by Government, and with an annual budget of around £445M, we support research and training in universities and strategically funded institutes. BBSRC research and the people we fund are helping society to meet major challenges, including food security, green energy and healthier, longer lives. Our investments underpin important UK economic sectors, such as farming, food, industrial biotechnology and pharmaceuticals.
5. About the Medical Research Council
For almost 100 years the Medical Research Council has improved the health of people in the UK and around the world by supporting the highest quality science. The MRC invests in world-class scientists. It has produced 29 Nobel Prize winners and sustains a flourishing environment for internationally recognised research. The MRC focuses on making an impact and provides the financial muscle and scientific expertise behind medical breakthroughs, including one of the first antibiotics penicillin, the structure of DNA and the lethal link between smoking and cancer. Today MRC funded scientists tackle research into the major health challenges of the 21st century. http://www.mrc.ac.uk
Article text (excluding photos or graphics) available under an Attribution-NonCommercial-ShareAlike Creative Commons license.
Photos and graphics subject to third party copyright used with permission or © Imperial College London.
Communications and Public Affairs