BibTex format
@article{Treanor:2006:10.1083/jcb.200601108,
author = {Treanor, B and Lanigan, PMP and Kumar, S and Dunsby, C and Munro, I and Auksorius, E and Culley, FJ and Purbhoo, MA and Phillips, D and Neil, MAA and Burshtyn, DN and French, PMW and Davis, DM},
doi = {10.1083/jcb.200601108},
journal = {Journal of Cell Biology},
pages = {153--161},
title = {Microclusters of inhibitory killer immunoglobulin like receptor signaling at natural killer cell immunological synapses},
url = {http://dx.doi.org/10.1083/jcb.200601108},
volume = {174},
year = {2006}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - We report the supramolecular organization of killer Ig–like receptor (KIR) phosphorylation using a technique applicable to imaging phosphorylation of any green fluorescent protein–tagged receptor at an intercellular contact or immune synapse. Specifically, we use fluorescence lifetime imaging (FLIM) to report Förster resonance energy transfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antibody. Visualization of KIR phosphorylation in natural killer (NK) cells contacting target cells expressing cognate major histocompatibility complex class I proteins revealed that inhibitory signaling is spatially restricted to the immune synapse. This explains how NK cells respond appropriately when simultaneously surveying susceptible and resistant target cells. More surprising, phosphorylated KIR was confined to microclusters within the aggregate of KIR, contrary to an expected homogeneous distribution of KIR signaling across the immune synapse. Also, yellow fluorescent protein–tagged Lck, a kinase important for KIR phosphorylation, accumulated in a multifocal distribution at inhibitory synapses. Spatial confinement of receptor phosphorylation within the immune synapse may be critical to how activating and inhibitory signals are integrated in NK cells.
AU - Treanor,B
AU - Lanigan,PMP
AU - Kumar,S
AU - Dunsby,C
AU - Munro,I
AU - Auksorius,E
AU - Culley,FJ
AU - Purbhoo,MA
AU - Phillips,D
AU - Neil,MAA
AU - Burshtyn,DN
AU - French,PMW
AU - Davis,DM
DO - 10.1083/jcb.200601108
EP - 161
PY - 2006///
SN - 1540-8140
SP - 153
TI - Microclusters of inhibitory killer immunoglobulin like receptor signaling at natural killer cell immunological synapses
T2 - Journal of Cell Biology
UR - http://dx.doi.org/10.1083/jcb.200601108
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000238940200014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL - 174
ER -