BibTex format
@article{Garcia:2016:10.1038/srep32417,
author = {Garcia, E and Hayden, A and Birts, C and Britton, E and Cowie, A and Pickard, K and Mellone, M and Choh, C and Derouet, M and Duriez, P and Noble, F and White, MJ and Primrose, JN and Strefford, JC and Rose-Zerilli, M and Thomas, GJ and Ang, Y and Sharrocks, AD and Fitzgerald, RC and Underwood, TJ and MacRae, S and Grehan, N and Abdullahi, Z and de, la Rue R and Noorani, A and Elliott, RF and de, Silva N and Bornschein, J and ODonovan, M and Contino, G and Yang, T-P and Chettouh, H and Crawte, J and Nutzinger, B and Edwards, PAW and Smith, L and Miremadi, A and Malhotra, S and Cluroe, A and Hardwick, R and Davies, J and Ford, H and Gilligan, D and Safranek, P and Hindmarsh, A and Sujendran, V and Carroll, N and Turkington, R and Hayes, SJ and Ang, Y and Preston, SR and Oakes, S and Bagwan, I and Save, V and Skipworth, RJE and Hupp, TR and ONeill, JR and Tucker, O and Taniere, P and Owsley, J and Crichton, C and Schusterreiter, C and Barr, H and Shepherd, N and Old, O and Lagergren, J a},
doi = {10.1038/srep32417},
journal = {Scientific Reports},
pages = {32417--32417},
title = {Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1},
url = {http://dx.doi.org/10.1038/srep32417},
volume = {6},
year = {2016}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - New biological tools are required to understand the functional significance of genetic events revealed by whole genome sequencing (WGS) studies in oesophageal adenocarcinoma (OAC). The MFD-1 cell line was isolated from a 55-year-old male with OAC without recombinant-DNA transformation. Somatic genetic variations from MFD-1, tumour, normal oesophagus, and leucocytes were analysed with SNP6. WGS was performed in tumour and leucocytes. RNAseq was performed in MFD-1, and two classic OAC cell lines FLO1 and OE33. Transposase-accessible chromatin sequencing (ATAC-seq) was performed in MFD-1, OE33, and non-neoplastic HET1A cells. Functional studies were performed. MFD-1 had a high SNP genotype concordance with matched germline/tumour. Parental tumour and MFD-1 carried four somatically acquired mutations in three recurrent mutated genes in OAC: TP53, ABCB1 and SEMA5A, not present in FLO-1 or OE33. MFD-1 displayed high expression of epithelial and glandular markers and a unique fingerprint of open chromatin. MFD-1 was tumorigenic in SCID mouse and proliferative and invasive in 3D cultures. The clinical utility of whole genome sequencing projects will be delivered using accurate model systems to develop molecular-phenotype therapeutics. We have described the first such system to arise from the oesophageal International Cancer Genome Consortium project.
AU - Garcia,E
AU - Hayden,A
AU - Birts,C
AU - Britton,E
AU - Cowie,A
AU - Pickard,K
AU - Mellone,M
AU - Choh,C
AU - Derouet,M
AU - Duriez,P
AU - Noble,F
AU - White,MJ
AU - Primrose,JN
AU - Strefford,JC
AU - Rose-Zerilli,M
AU - Thomas,GJ
AU - Ang,Y
AU - Sharrocks,AD
AU - Fitzgerald,RC
AU - Underwood,TJ
AU - MacRae,S
AU - Grehan,N
AU - Abdullahi,Z
AU - de,la Rue R
AU - Noorani,A
AU - Elliott,RF
AU - de,Silva N
AU - Bornschein,J
AU - ODonovan,M
AU - Contino,G
AU - Yang,T-P
AU - Chettouh,H
AU - Crawte,J
AU - Nutzinger,B
AU - Edwards,PAW
AU - Smith,L
AU - Miremadi,A
AU - Malhotra,S
AU - Cluroe,A
AU - Hardwick,R
AU - Davies,J
AU - Ford,H
AU - Gilligan,D
AU - Safranek,P
AU - Hindmarsh,A
AU - Sujendran,V
AU - Carroll,N
AU - Turkington,R
AU - Hayes,SJ
AU - Ang,Y
AU - Preston,SR
AU - Oakes,S
AU - Bagwan,I
AU - Save,V
AU - Skipworth,RJE
AU - Hupp,TR
AU - ONeill,JR
AU - Tucker,O
AU - Taniere,P
AU - Owsley,J
AU - Crichton,C
AU - Schusterreiter,C
AU - Barr,H
AU - Shepherd,N
AU - Old,O
AU - Lagergren,J
AU - Gossage,J
AU - Davies,A
AU - Chang,F
AU - Zylstra,J
AU - Sanders,G
AU - Berrisford,R
AU - Harden,C
AU - Bunting,D
AU - Lewis,M
AU - Cheong,E
AU - Kumar,B
AU - Parsons,SL
AU - Soomro,I
AU - Kaye,P
AU - Saunders,J
AU - Lovat,L
AU - Haidry,R
AU - Eneh,V
AU - Igali,L
AU - Welch,I
AU - Scott,M
AU - Sothi,S
AU - Suortamo,S
AU - Lishman,S
AU - Beardsmore,D
AU - Anderson,C
AU - Smith,ML
AU - Secrier,M
AU - Eldridge,MD
AU - Bower,L
AU - Achilleos,A
AU - Lynch,AG
AU - Tavare,S
DO - 10.1038/srep32417
EP - 32417
PY - 2016///
SN - 2045-2322
SP - 32417
TI - Authentication and characterisation of a new oesophageal adenocarcinoma cell line: MFD-1
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/srep32417
VL - 6
ER -