Centre technical experts
Reuben Yaa
Postdoc – Nott lab
Marianna Papageorgopoulou
Research Assistant – Matthews Lab
The FACSMelody is a 9 colour high speed cell sorter with three lasers (Violet 405nm, Blue 488nm and Red 640nm), a fixed optical alignment and a pressure-driven fluidics system.
100µm nozzle size that runs at 23 psi and 34 kHz providing 34,000 drops per second and a maximum sort speed of around 11,000 ev/s.
The system is equipped with a versatile sort collection chamber that allows users to sort into up to 4-way tubes or 96 well plates. Cooling and heating for sort collection are available.
Forward and side scatter properties (FSC and SSC) allow for a minimum detectable particle size of 0.5 μm. The maximum data acquisition rate is 70,000 events/second.
|
Blue laser (488nm) |
Filter |
Mirror |
|
Side Scatter |
488/15 |
NA |
|
BD Horizon Brilliant Blue 515, FITC, Alexa Fluor 488, GFP |
527/32 |
507 LP |
|
PE, PI |
586/42 |
560 LP |
|
PerCP, PerCP-Cy5.5, 7-AAD |
700/54 |
665 LP |
|
PE-Cy7 |
695/40 |
752 LP |
|
Red Laser (640nm) |
Filter |
Mirror |
|
APC, Alexa Fluor 647 |
660/10 |
NA |
|
APC-Cy7, APC-H7 |
783/56 |
752 LP |
|
Violet Laser (405nm) |
Filter |
Mirror |
|
BD Horizon Brilliant Violet 421, V450, Pacific Blue, DAPI |
448/45 |
NA |
|
BD Horizon Brilliant Violet 510, V500, AmCyan |
528/45 |
500 LP |
|
BD Horizon Brilliant Violet 786 |
none |
755 LP |
*Note: This system is in a safety cabinet so Class 2 work may take place, risk assessment must be done first.
The cost of running consumables will be recharged to users quarterly.
