BibTex format

author = {Low, WW and Wong, J and Beltran, L and Seddon, C and David, S and Kwong, H-S and Bizeau, T and Wang, F and Pena, A and Costa, TRD and Pham, B and Chen, M and Egelman, E and Beis, K and Frankel, G},
doi = {10.1038/s41564-022-01146-4},
journal = {Nature Microbiology},
pages = {1016--1027},
title = {Mating pair stabilization mediates bacterial conjugation species specificity},
url = {},
volume = {7},
year = {2022}

RIS format (EndNote, RefMan)

AB - Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating thespread of virulence and resistance plasmids. Here we describe how variants of the plasmid-encoded donor outer membrane(OM) protein TraN cooperate with distinct OM receptors in recipients to mediate mating pair stabilization and efficient DNAtransfer. We show that TraN from the plasmids pKpQIL (Klebsiella pneumoniae), R100-1 (Shigella flexneri) and pSLT (SalmonellaTyphimurium), and the prototypical F plasmid (Escherichia coli) interact with OmpK36, OmpW and OmpA, respectively.Cryo-EM analysis revealed that TraN pKpQIL interacts with OmpK36 through the insertion of a β-hairpin in the tip of TraN intoa monomer of the OmpK36 trimer. Combining bioinformatic analysis with AlphaFold structural predictions, we identified afourth TraN structural variant that mediates mating pair stabilization by binding OmpF. Accordingly, we devised a classifica-tion scheme for TraN homologues on the basis of structural similarity and their associated receptors: TraNα (OmpW), TraNβ(OmpK36), TraNγ (OmpA), TraNδ (OmpF). These TraN-OM receptor pairings have real-world implications as they reflect thedistribution of resistance plasmids within clinical Enterobacteriaceae isolates, demonstrating the importance of mating pairstabilization in mediating conjugation species specificity. These findings will allow us to predict the distribution of emergingresistance plasmids in high-risk bacterial pathogens.
AU - Low,WW
AU - Wong,J
AU - Beltran,L
AU - Seddon,C
AU - David,S
AU - Kwong,H-S
AU - Bizeau,T
AU - Wang,F
AU - Pena,A
AU - Costa,TRD
AU - Pham,B
AU - Chen,M
AU - Egelman,E
AU - Beis,K
AU - Frankel,G
DO - 10.1038/s41564-022-01146-4
EP - 1027
PY - 2022///
SN - 2058-5276
SP - 1016
TI - Mating pair stabilization mediates bacterial conjugation species specificity
T2 - Nature Microbiology
UR -
UR -
UR -
VL - 7
ER -