@article{Kim:2015:10.1371/journal.pbio.1002111,
author = {Kim, YH and Larsen, HL and Rue, P and Lemaire, LA and Ferrer, J and Grapin-Botton, A},
doi = {10.1371/journal.pbio.1002111},
journal = {PLOS Biology},
title = {Cell Cycle-Dependent Differentiation Dynamics Balances Growth and Endocrine Differentiation in the Pancreas},
url = {http://dx.doi.org/10.1371/journal.pbio.1002111},
volume = {13},
year = {2015}
}

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TY  - JOUR
AB  - Organogenesis relies on the spatiotemporal balancing of differentiation and proliferationdriven by an expanding pool of progenitor cells. In the mouse pancreas, lineage tracing atthe population level has shown that the expanding pancreas progenitors can initially giverise to all endocrine, ductal, and acinar cells but become bipotent by embryonic day 13.5,giving rise to endocrine cells and ductal cells. However, the dynamics of individual progenitorsbalancing self-renewal and lineage-specific differentiation has never been described.Using three-dimensional live imaging and in vivo clonal analysis, we reveal the contributionof individual cells to the global behaviour and demonstrate three modes of progenitor divisions:symmetric renewing, symmetric endocrinogenic, and asymmetric generating a progenitorand an endocrine progenitor. Quantitative analysis shows that the endocrinedifferentiation process is consistent with a simple model of cell cycle–dependent stochasticpriming of progenitors to endocrine fate. The findings provide insights to define control parametersto optimize the generation of β-cells in vitro.
AU  - Kim,YH
AU  - Larsen,HL
AU  - Rue,P
AU  - Lemaire,LA
AU  - Ferrer,J
AU  - Grapin-Botton,A
DO  - 10.1371/journal.pbio.1002111
PY  - 2015///
SN  - 1545-7885
TI  - Cell Cycle-Dependent Differentiation Dynamics Balances Growth and Endocrine Differentiation in the Pancreas
T2  - PLOS Biology
UR  - http://dx.doi.org/10.1371/journal.pbio.1002111
UR  - http://hdl.handle.net/10044/1/26527
VL  - 13
ER  - 

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