HWF1 - Zeiss Axio Observer

A Zeiss Axio Observer inverted microscope with a fully motorised stage, controlled by Zen acquisition software. This system is also equipped with a large range of filter sets, LED light sources from UV to far-red, and a highly sensitive camera for fast, low-light imaging and a colour camera for standard histology.

Advantages of this microscope

• multi-Channel, Z-Stack, Timelapse, Tiles & Positions and Autofocus including Definite Focus - focus holding system for long term timelapse experiments
• Colibri.2 LED for Fast Excitation switching, capable of switching excitation wavelengths in less than 1ms with fast acquisition components
• Slide, dish and multiwell formats
• Live cell environmental control system including Temperature and CO2 control
• Colour camera for histology imaging.
• Hamamatsu Flash 4.0 fast camera

Location

Hammersmith Hospital, L-Block room 521

Quickstart guide

Quickstart guide - HWF1 Zeiss Cell Observer (PDF)‌

HWF1 Live Imaging Guide (PDF)

Widefield Tiling Method (PDF)

Sample Carrier Setup (PDF)

Multi-position acquisition (PDF)

Tiling using Colour Camera (PDF)

Stitching in ZEN Blue (PDF)

Hardware configuration

Please see the FILM wiki page - HWF1 hardware configuration

Flash Camera Data (PDF)

Frame Size Calculator (XLS)

HWF1 Filter and LED Setup (PDF)‌

Zeiss Objective List (PDF)

Offline software

• ZEN Lite (requires Registration)
• FIJI