Leica SP8 - STELLARIS 5 Inverted Light Sheet Confocal Microscope

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Advantages of this microscope

  • Live confocal imaging with high sensitivity
  • 4 internal detection channels equipped with Power HyD S
  •  6 Laser (Solid State) - 405, 448, 488, 514, 561, 638nm
  • White Light Laser 485-685nm Super Continuum - Tau Sense Modality
  • Motorised stage - slide, dish and multiwell formats
  • Z-Galvanometer Stage
  • Fully Integrated Light Sheet modality
  • sCMOS Camera - DFC9000 GTC DLS

Confocal Objectives

  • HC PL APO 10x/0.40 CS2
  • HC PL APO 20x/0.75 CS2
  • HC PL APO 40x/1.30 Oil CS2
  • HC PL APO 63x/1.40 OIL CS2

Light Sheet Objectives

  • HC PL FLUOTAR 5x/0.15 IMM DLS
  • HC APO L10x/0.30 W DLS
  • HC APO L 20x/0.50 W DLS
  • HC FLUOTAR L25x/0.95 W DLS

Working distances

Availible combinations of mirror cap (TwinFlect) and objectives for use in lightsheet experiments

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Location

Sir Alexander Fleming building, SAF 410


Quickstart guides

 Quickstart guide Confocal LS1 (pdf)

Tiling and Multi-position Imaging (PDF)

Quickstart guide - Light Sheet LS1 Leica Stellaris Inverted (PDF)

Sample Preparation

Light Sheet Sample Preparation (PDF)

Using U-Shaped Glass Capillaries for Sample Mounting

LS1 Live Imaging Guide (PDF)

Leica Webinar

The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy

Leica Objectives


Offline software