Location: Hammersmith campus, Victor Phillip Dahdaleh building, 2nd floor, lab VPD 206A.
This sorter is operated with 70um, 85um, or 100um nozzle. The model also fits 130um nozzle (suitable for very large and/or fragile cells), but the facility does not currently offer this option.
Can sort up to 4-way into tubes, and also sorts into 96-well plates.
Filter configuration:
| Laser | Mirror | Filter | Common fluorochromes, dyes, fluorescent proteins |
| 355nm UV | 450LP | 515/30 | BUV496, LD Aqua (DAPI, Hoechst 33342, Zombie UV, LD Blue) |
| - | 379/28 | BUV395 | |
| 405nm Violet | 750LP | 780/60 | BV750, BV785 |
| 690LP | 710/50 | BV711 | |
| 630LP | 660/20 | BV650 | |
| 595LP | 610/20 | BV605 | |
| 505LP | 525/50 | Zombie Aqua, BV510, BV480 | |
| - | 450/50 | DAPI, BV421, Pacific Blue, eF450, BFP, LD Violet | |
| 488nm Blue | 685LP | 710/50 | PerCP-Cy5.5, PerCP-eF710, PE-Cy5.5, BB700, RB705 |
| 505LP | 530/30 | FITC, AF488, GFP, BB515 | |
| 561nm Yellow | 735LP | 780/60 | PE-Cy7 |
| 685LP | 710/50 | PerCP-Cy5.5, PerCP-eF710, BB700 | |
| 630LP | 670/25 | PE-Cy5, 7-AAD | |
| 600LP | 610/20 | PE-TexasRed, PE-CF594, mCherry | |
| - | 582/15 | PE, tdTomato | |
| 640nm Red | 750LP | 780/60 | APCFire750, APCFire810, AF750, APC-Cy7, APC-eF780 |
| 690LP | 730/45 | AF680, AF700, DRAQ5, DRAQ7 | |
| - | 670/30 | APC, AF647, TO-PRO-3 |
Some spare filters are available and, depending on the full panel, it might be possible to use BUV563, BUV615, BUV661, BUV737, BUV805, etc. Please enquire with staff.
Compensation can be calculated automatically or manually based on single-stained controls and can also be edited manually.
Software: BD FACSDiva
Manufacturer website: BD FACSAria Fusion
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk