Location: Hammersmith campus, Commonwealth building, 8th floor, lab CWB 8N4.
This sorter is only operated with 70um nozzle. The model also fits 85um, 100um, and 130um nozzles, but the facility does not currently offer these options.
Can sort up to 4-way into tubes, and also sorts into 96-well plates.
Filter configuration:
| Laser | Long-pass | Band-pass | Common fluorochromes, dyes, fluorescent proteins |
| 407nm Violet | 595LP | V610/20 | BV605 |
| 505LP | V525/50 | Zombie Aqua, BV510, BV480 | |
| - | V450/40 | BV421, Pacific Blue, eF450, DAPI | |
| 488nm Blue | 755LP | B780/60 | PE-Cy7 |
| 685LP | B710/50 | PerCP-Cy5.5, PerCP-eF710, BB700 | |
| 635LP | B670/14 | PerCP, PE-Cy5, 7-AAD | |
| 600LP | B610/20 | PE-TexasRed, PE-CF594, mCherry | |
| 550LP | B575/25 | PE, tdTomato | |
| 505LP | B525/50 | AF488, FITC, BB515, GFP, YFP, mVenus | |
| 633nm Red | 755LP | R780/60 | APCFire750, APCFire810, AF750, APC-Cy7, APC-eF780 |
| 690LP | R710/50 | AF680, AF700, DRAQ5, DRAQ7 | |
| - | R670/14 | APC, AF647, TO-PRO-3 |
Some spare filters are available and, depending on the full panel, it might be possible to use BV570, BV650, BV711, BV785, etc. Please enquire with staff.
Compensation can be calculated automatically or manually based on single-stained controls and can also be edited manually.
Software: BD FACSDiva
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk