Location: Hammersmith campus, Victor Phillip Dahdaleh (VPD) building, 2nd floor, lab VPD 206A.
In addition to staff-assisted cell sorting services, this sorter can also be used autonomously after training.
This sorter model fits only 100um nozzle.
Can sort up to 4-way into tubes, and also sorts into 96-well and 384-well plates.
Filter configuration:
| Laser | Mirror | Filter | Common fluorochromes, dyes, fluorescent proteins |
| 405nm Violet | 755LP | - | BV785, BV786 |
| 500LP | 528/45 | AmCyan, BV510, V500 | |
| 448/45 | - | DAPI, Pacific Blue, BV421, V450 | |
| 488nm Blue | 752LP | 783/56 | PE-Cy7 |
| 665LP | 700/54 | PerCP-eF710, PerCP-Cy5.5, Per-CP, PE-Cy5, 7-AAD, BB700 | |
| 560LP | 586/42 | PE, PI, PE-CF594, PE-TexasRed | |
| 507LP | 527/32 | FITC, AF488, GFP, BB515 | |
| 640nm Red | 752LP | 783/56 | APC-Cy7, APC-eF780, APC-H7 |
| 660/10 | 660/10 | APC, AF647 |
No spare filters are available, but some fluorophores are detectable with sub-optimal filters. Please enquire with staff.
Compensation can only be calculated automatically based on single-stained controls. It cannot be calculated or edited manually.
Software: BD FACSChorus
Manufacturer website: BD FACSMelody Cell Sorter
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk