Location: Hammersmith campus, Victor Phillip Dahdaleh building, 2nd floor, lab VPD 206.
Filter configuration:
| Laser | Long-pass | Band-pass | Parameter name in DIVA | Common fluorochromes | Notes * |
| 355nm UV |
505 | 530/30 | 355-530/30 | BUV496 | For BUV737 can use 690LP and 740/35. For BUV805 can use 690LP and 780/60 |
| - | 450/50 | 355-450/50 | DAPI, Hoechst, LD Blue, Zombie UV | For BUV395 can use 379/28 | |
| 405nm Violet |
750 | 780/60 | 405-780/60 | BV785 | |
| 690 | 710/50 | 405-710/50 | BV711 | ||
| 630 | 655/8 | 405-655/8 | BV650 | ||
| 595 | 605/12 | 405-605/12 | BV605 | For BV570 can use 575LP and 585/15 | |
| 475 | 525/50 | 405-525/50 | BV480, BV510, Pacific Orange, Zombie Aqua, LD Lime, LD Yellow | ||
| - | 450/50 | 405-450/50 | DAPI, BV421, Pacific Blue, eF450 | ||
| 488nm Blue |
685 | 710/50 | 488-710/50 | PerCP-Cy5.5, PerCP-eF710, BB700 | |
| 655 | 675/20 | 488-675/20 | PerCP, PECy-5 | ||
| 505 | 530/30 | 488-530/30 | FITC, AF488, GFP, YFP, Thiazole Orange, CFSE, LD Green | ||
| 561nm Yellow/Green |
750 | 780/60 | 561-780/60 | PE-Cy7 | |
| 685 | 710/50 | 561-710/50 | PE-Cy5.5, 7-AAD | ||
| 600 | 620/10 | 561-620/10 | PE-Dazzle594, PE-CF594, PE-TexasRed, mCherry, Propidium Iodide | ||
| 570 | 582/15 | 561-582/15 | PE, tdTomato, RFP | ||
| 640nm Red |
750 | 780/60 | 640-780/60 | APC-Cy7, APC-eF780, LD NIR780 | |
| 710 | 720/40 | 640-720/40 | AF700 | ||
| - | 670/14 | 640-670/14 | APC, AF647, DRAQ5, TO-PRO-3, LD FarRed |
Spare filters available. Please enquire with staff.
Equipped with BD High Throughput Sampler (HTS) for 96-well plate readouts.
Manufacturer website: BD LSRFortessa
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk