Location: Hammersmith campus, Commonwealth building, 9th floor, lab CWB 9N3.
Filter configuration:
| Laser | Long-pass | Band-pass | Parameter name in DIVA | Common fluorochromes |
| 405nm Violet |
750LP | 780/60 | V780/60 | BV785 |
| 685LP | 710/50 | V710/50 | BV711 | |
| 635LP | 670/30 | V670/30 | BV650 | |
| 600LP | 610/20 | V610/20 | BV605 | |
| 505LP | 525/50 | V525/50 | BV480, BV510, Pacific Orange, Zombie Aqua, LD Lime, LD Yellow | |
| - | 450/50 | V450/50 | DAPI, BV421, Pacific Blue, eF450, BFP, LD Violet | |
| 488nm Blue |
685LP | 710/50 | B710/50 | PerCP, PerCP-Cy5.5, PerCP-eF710, PE-Cy5.5, BB700, RB705 |
| 505LP | 530/30 | B530/30 | FITC, AF488, GFP, YFP, Thiazole Orange, CFSE, LD Green | |
| 561nm Yellow/Green |
750LP | 780/60 | YG780/60 | PE-Cy7 |
| 685LP | 710/50 | YG710/50 | PE-Cy5.5 | |
| 635LP | 670/30 | YG670/30 | PE-Cy5, 7-AAD | |
| 600LP | 610/20 | YG610/20 | PE-Dazzle594, PE-CF594, PE-TexasRed, mCherry, Propidium Iodide | |
| - | 585/15 | YG585/15 | PE, tdTomato, RFP | |
| 640nm Red |
750LP | 780/60 | R780/60 | APC-Cy7, APC-eF780, LD NIR780 |
| 690LP | 730/45 | R730/45 | AF700 | |
| - | 670/30 | R670/30 | APC, AF647, DRAQ5, TO-PRO-3, LD FarRed |
Equipped with BD High Throughput Sampler (HTS) for 96-well plate readouts.
Manufacturer website: LSRFortessa X-20
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk