Location: Hammersmith campus, Victor Phillip Dahdaleh (VPD) building, 2nd floor, lab VPD 206.
Can detect particles in the 7nm to 1000 nm range, but works best in the 10nm to 100nm range for gold nanoparticles, and in the 40nm to 200nm range for biological particles.
This instrument is equipped with two collinear excitation lasers: blue (488nm) and red (640nm). It measures particle sizes and quantifies concentration based on light side scatter (SSC) from the blue laser. A maximum of 2 fluorescence emission signals can also be detected from fluorophores excited by the blue and/or red laser.
| Detector | Dichroic mirror | Band-pass filter | Channel |
| SPCM-1 | - | 488/10 (fixed) | SSC |
| SPCM-2 | DicF495 | 525/40 (fixed) | FITC, AF488, GFP |
| SPCM-3 | DicF555 | 3 options * | 3 options * |
| * Options: | 580/40 | PE, AF532, AF555, AF561 | |
| 670/30 | APC, AF647, PE-Cy5, PerCP | ||
| 710/40 | PerCP-Cy5.5, PerCP-eF710 |
Manufacturer website: NanoFCM Flow Nanoanalyzer
Useful links
General enquiries
Hammersmith Flow Cytometry Facility
Dr Victor Phillip Dahdaleh (VPD) building
Level 2, Lab 206 and Office 205
Hammersmith Hospital
Du Cane Road
London W12 0NN
Facility Managers
Joana De Teixeira Carrelha
Email: j.de-teixeira-carrelha@imperial.ac.uk
Fredrik Wållberg
Email: f.wallberg@imperial.ac.uk