@article{Moffatt:2017:10.7861/clinmedicine.17-6-525,
author = {Moffatt, MF and Cookson, WOCM},
doi = {10.7861/clinmedicine.17-6-525},
journal = {Clinical Medicine},
pages = {525--529},
title = {The lung microbiome in health and disease},
url = {http://dx.doi.org/10.7861/clinmedicine.17-6-525},
volume = {17},
year = {2017}
}

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TY  - JOUR
AB  - The Human Microbiome Project began 10 years ago, leadingto a signifi cant growth in understanding of the role the humanmicrobiome plays in health and disease. In this article, weexplain with an emphasis on the lung, the origins of microbiomeresearch. We discuss how 16S rRNA gene sequencingbecame the fi rst major molecular tool to examine the bacterialcommunities present within the human body. We highlightthe pitfalls of molecular-based studies, such as false fi ndingsresulting from contamination, and the limitations of 16S rRNAgene sequencing. Knowledge about the lung microbiome hasevolved from initial scepticism to the realisation that it mighthave a signifi cant infl uence on many illnesses. We also discussthe lung microbiome in the context of disease by givingexamples of important respiratory conditions. In addition, wedraw attention to the challenges for metagenomic studies ofrespiratory samples and the importance of systematic bacterialisolation to enable host–microbiome interactions to beunderstood. We conclude by discussing how knowledge of thelung microbiome impacts current clinical diagnostics.
AU  - Moffatt,MF
AU  - Cookson,WOCM
DO  - 10.7861/clinmedicine.17-6-525
EP  - 529
PY  - 2017///
SN  - 1470-2118
SP  - 525
TI  - The lung microbiome in health and disease
T2  - Clinical Medicine
UR  - http://dx.doi.org/10.7861/clinmedicine.17-6-525
UR  - http://hdl.handle.net/10044/1/56116
VL  - 17
ER  - 

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