BibTex format
@article{Mourier:2022:10.1038/s41467-022-28287-8,
author = {Mourier, T and Shuaib, M and Hala, S and Mfarrej, S and Alofi, F and Naeem, R and Alsomali, A and Jorgensen, D and Subudhi, AK and Ben, Rached F and Guan, Q and Salunke, RP and Ooi, A and Esau, L and Douvropoulou, O and Nugmanova, R and Perumal, S and Zhang, H and Rajan, I and Al-Omari, A and Salih, S and Shamsan, A and Al, Mutair A and Taha, J and Alahmadi, A and Khotani, N and Alhamss, A and Mahmoud, A and Alquthami, K and Dageeg, A and Khogeer, A and Hashem, AM and Moraga, P and Volz, E and Almontashiri, N and Pain, A},
doi = {10.1038/s41467-022-28287-8},
journal = {Nature Communications},
title = {SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load},
url = {http://dx.doi.org/10.1038/s41467-022-28287-8},
volume = {13},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 SARS-CoV-2 genomes collected from patients in Saudi Arabia from March to August 2020. We show that two consecutive mutations (R203K/G204R) in the nucleocapsid (N) protein are associated with higher viral loads in COVID-19 patients. Our comparative biochemical analysis reveals that the mutant N protein displays enhanced viral RNA binding and differential interaction with key host proteins. We found increased interaction of GSK3A kinase simultaneously with hyper-phosphorylation of the adjacent serine site (S206) in the mutant N protein. Furthermore, the host cell transcriptome analysis suggests that the mutant N protein produces dysregulated interferon response genes. Here, we provide crucial information in linking the R203K/G204R mutations in the N protein to modulations of host-virus interactions and underline the potential of the nucleocapsid protein as a drug target during infection.
AU - Mourier,T
AU - Shuaib,M
AU - Hala,S
AU - Mfarrej,S
AU - Alofi,F
AU - Naeem,R
AU - Alsomali,A
AU - Jorgensen,D
AU - Subudhi,AK
AU - Ben,Rached F
AU - Guan,Q
AU - Salunke,RP
AU - Ooi,A
AU - Esau,L
AU - Douvropoulou,O
AU - Nugmanova,R
AU - Perumal,S
AU - Zhang,H
AU - Rajan,I
AU - Al-Omari,A
AU - Salih,S
AU - Shamsan,A
AU - Al,Mutair A
AU - Taha,J
AU - Alahmadi,A
AU - Khotani,N
AU - Alhamss,A
AU - Mahmoud,A
AU - Alquthami,K
AU - Dageeg,A
AU - Khogeer,A
AU - Hashem,AM
AU - Moraga,P
AU - Volz,E
AU - Almontashiri,N
AU - Pain,A
DO - 10.1038/s41467-022-28287-8
PY - 2022///
SN - 2041-1723
TI - SARS-CoV-2 genomes from Saudi Arabia implicate nucleocapsid mutations in host response and increased viral load
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-022-28287-8
UR - https://www.ncbi.nlm.nih.gov/pubmed/35105893
VL - 13
ER -