In this section
@article{Lallier:1996:10.1242/dev.122.8.2539,
author = {Lallier, TE and Whittaker, CA and DeSimone, DW},
doi = {10.1242/dev.122.8.2539},
journal = {Development},
pages = {2539--2554},
title = {Integrin alpha 6 expression is required for early nervous system development in Xenopus laevis.},
url = {http://dx.doi.org/10.1242/dev.122.8.2539},
volume = {122},
year = {1996}
}
TY - JOUR
AB - The integrin alpha 6 subunit pairs with both the beta 1 and beta 4 subunits to form a subfamily of laminin receptors. Here we report the cDNA cloning and primary sequence for the Xenopus homologue of the mammalian integrin alpha 6 subunit. We present data demonstrating the spatial and temporal expression of alpha 6 mRNA and protein during early development. Initially, alpha 6 transcripts are expressed in the dorsal ectoderm and future neural plate at the end of gastrulation. Later in development, alpha 6 mRNAs are expressed in a variety of neural derivatives, including the developing sensory placodes (otic and olfactory) and commissural neurons within the neural tube. Integrin alpha 6 is also expressed in the elongating pronephric duct as well as a subset of the rhombencephalic neural crest, which will form the Schwann cells lining several cranial nerves (VII, VIII and X). In vivo expression of an alpha 6 antisense transcript in the animal hemisphere leads to a reduction in alpha 6 protein expression, a loss of adhesion to laminin, and severe defects in normal development. In 35% of cases, reduced levels of alpha 6 expression result in embryos that complete gastrulation normally but arrest at neurulation prior to the formation of the neural plate. In an additional 22% of cases, embryos develop with severe axial defects, including complete loss of head or tail structures. In contrast, overexpression of the alpha 6 subunit by injection of full-length mRNA has no apparent effect on embryonic development. Co-injection of antisense and sense plasmid constructs results in a partial rescue of the antisense-generated phenotypes. These data indicate that the integrin alpha 6 subunit is critical for the early development of the nervous system in amphibians.
AU - Lallier,TE
AU - Whittaker,CA
AU - DeSimone,DW
DO - 10.1242/dev.122.8.2539
EP - 2554
PY - 1996///
SN - 0950-1991
SP - 2539
TI - Integrin alpha 6 expression is required for early nervous system development in Xenopus laevis.
T2 - Development
UR - http://dx.doi.org/10.1242/dev.122.8.2539
UR - https://www.ncbi.nlm.nih.gov/pubmed/8756298
VL - 122
ER -
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