BibTex format
@inbook{Vogels:2023:10.1201/9781003298038-30,
author = {Vogels, CBF and Breban, MI and Ott, IM and Alpert, T and Petrone, ME and Watkins, AE and Kalinich, CC and Earnest, R and Rothman, JE and de, Jesus JG and Claro, IM and Ferreir, GM and Crispim, MAE and Singh, L and Tegally, H and Anyaneji, UJ and Hodcrof, EB and Mason, CE and Khullar, G and Metti, J and Dudley, JT and MacKay, MJ and Nash, M and Wang, J and Liu, C and Hui, P and Murphy, S and Neal, C and Laszlo, E and Landry, ML and Muyombwe, A and Downing, R and Razeq, J and de, Oliveira T and Faria, NR and Sabino, EC and Neher, RA and Fauver, JR and Grubaugh, ND},
booktitle = {Advances in Medical Imaging Detection and Diagnosis},
doi = {10.1201/9781003298038-30},
pages = {891--905},
title = {Multiplex qPCR Discriminates Variants of Concern to Enhance Global Surveillance of SARS-CoV-2},
url = {http://dx.doi.org/10.1201/9781003298038-30},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - CHAP
AB - Broadly accessible and inexpensive surveillance methods are needed to track Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOC) around the world. While sequencing is the gold standard to identify circulating SARS-CoV-2 variants, routine genomic surveillance is not available in many locations primarily due to a lack of resources and expertise. The Institutional Review Board from the Yale University Human Research Protection Program determined that the RT-qPCR testing and sequencing of de-identified remnant COVID-19 clinical samples conducted in this study is not research involving human patients. Multiplex-PCR products were purified by using AmpureXP beads, and quantification was carried out using the Qubit dsDNA High Sensitivity assay on the Qubit 3.0.
AU - Vogels,CBF
AU - Breban,MI
AU - Ott,IM
AU - Alpert,T
AU - Petrone,ME
AU - Watkins,AE
AU - Kalinich,CC
AU - Earnest,R
AU - Rothman,JE
AU - de,Jesus JG
AU - Claro,IM
AU - Ferreir,GM
AU - Crispim,MAE
AU - Singh,L
AU - Tegally,H
AU - Anyaneji,UJ
AU - Hodcrof,EB
AU - Mason,CE
AU - Khullar,G
AU - Metti,J
AU - Dudley,JT
AU - MacKay,MJ
AU - Nash,M
AU - Wang,J
AU - Liu,C
AU - Hui,P
AU - Murphy,S
AU - Neal,C
AU - Laszlo,E
AU - Landry,ML
AU - Muyombwe,A
AU - Downing,R
AU - Razeq,J
AU - de,Oliveira T
AU - Faria,NR
AU - Sabino,EC
AU - Neher,RA
AU - Fauver,JR
AU - Grubaugh,ND
DO - 10.1201/9781003298038-30
EP - 905
PY - 2023///
SP - 891
TI - Multiplex qPCR Discriminates Variants of Concern to Enhance Global Surveillance of SARS-CoV-2
T1 - Advances in Medical Imaging Detection and Diagnosis
UR - http://dx.doi.org/10.1201/9781003298038-30
ER -