BibTex format
@article{Delma:2025:10.3201/eid3107.241918,
author = {Delma, FZ and Spruijtenburg, B and Meis, JF and de, Jong AW and Groot, J and Rhodes, J and Melchers, WJG and Verweij, PE and de, Groot T and Meijer, EFJ and Buil, JB},
doi = {10.3201/eid3107.241918},
journal = {Emerging Infectious Diseases},
pages = {1354--1364},
title = {Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands},
url = {http://dx.doi.org/10.3201/eid3107.241918},
volume = {31},
year = {2025}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Candida tropicalis is the second most virulent Candida species after C. albicans. Previous studies from the Netherlands and France reported a notable reduction in susceptibility to flucytosine (5-FC) in a substantial proportion of C. tropicalis isolates. We investigated epidemiologic patterns of C. tropicalis isolates in the Netherlands and the genetic mechanisms driving widespread non–wild-type (WT) 5-FC resistance. We conducted antifungal susceptibility testing and used advanced molecular techniques, including short tandem repeat genotyping and whole-genome sequencing paired with single-nucleotide polymorphism analysis, to analyze 250 C. tropicalis isolates collected across the Netherlands during 2012–2022. Our findings revealed the rapid emergence of a 5-FC–resistant, non-WT C. tropicalis clade, accounting for >40% of all C. tropicalis isolates by 2022. Genomic analysis identified a homozygous nonsense mutation in the FCY2 gene, which was exclusive to this non-WT population. Continued surveillance efforts are needed to detect and prevent the spread of drug-resistant Candida species.
AU - Delma,FZ
AU - Spruijtenburg,B
AU - Meis,JF
AU - de,Jong AW
AU - Groot,J
AU - Rhodes,J
AU - Melchers,WJG
AU - Verweij,PE
AU - de,Groot T
AU - Meijer,EFJ
AU - Buil,JB
DO - 10.3201/eid3107.241918
EP - 1364
PY - 2025///
SN - 1080-6040
SP - 1354
TI - Emergence of Flucytosine-Resistant Candida tropicalis Clade, the Netherlands
T2 - Emerging Infectious Diseases
UR - http://dx.doi.org/10.3201/eid3107.241918
UR - https://doi.org/10.3201/eid3107.241918
VL - 31
ER -