BibTex format
@article{Gifford:2025:10.1038/s42003-025-09321-z,
author = {Gifford, H and Bedekovic, T and Helmstetter, N and Gregory, J and Ma, Q and Brand, AC and Wilson, D and Rhodes, J and Ramsdale, M and Kudoh, T and Farrer, RA},
doi = {10.1038/s42003-025-09321-z},
journal = {Commun Biol},
title = {Xenosiderophore transporter gene expression and clade-specific filamentation in Candida auris killifish (Aphanius dispar) infection.},
url = {http://dx.doi.org/10.1038/s42003-025-09321-z},
volume = {8},
year = {2025}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Candida auris is a critical priority fungal pathogen (World Health Organization). Clinical management is challenging, with high associated mortality, rapidly increasing antifungal resistance, and frequent nosocomial outbreaks. A critical bottleneck in understanding virulence is the lack of gene expression profiling models during infection. We developed a fish embryo yolk-sac microinjection model using Aphanius dispar (Arabian killifish; AK) at human body temperature. This enabled interrogation of infection dynamics via dual host-pathogen RNA-seq across five major clades of C. auris (I-V). Host responses included heat shock, complement activation, and nutritional immunity, notably haem oxygenase (HMOX) expression during clade IV infection. We identified a pathogen transcriptional signature across all five clades, strongly enriched for putative xenosiderophore transmembrane transporter candidate (XTC) genes. We describe this family and a sub-clade of five putative haem transport-related (HTR) genes. Only the basal clade V isolate formed filaments, associated with canonical and atypical regulators of morphogenesis. Clades I and IV demonstrated increased virulence, accompanied by up-regulation of three HTR genes in clade IV, and the non-mating mating-type locus (MTL) gene PIKA in both clades. Our study provides insights into C. auris pathogenesis, highlighting species-wide in vivo up-regulation of XTC genes during host tissue infection.
AU - Gifford,H
AU - Bedekovic,T
AU - Helmstetter,N
AU - Gregory,J
AU - Ma,Q
AU - Brand,AC
AU - Wilson,D
AU - Rhodes,J
AU - Ramsdale,M
AU - Kudoh,T
AU - Farrer,RA
DO - 10.1038/s42003-025-09321-z
PY - 2025///
TI - Xenosiderophore transporter gene expression and clade-specific filamentation in Candida auris killifish (Aphanius dispar) infection.
T2 - Commun Biol
UR - http://dx.doi.org/10.1038/s42003-025-09321-z
UR - https://www.ncbi.nlm.nih.gov/pubmed/41419766
VL - 8
ER -