@article{Macé:2022:10.1038/s41586-022-04859-y,
author = {Macé, K and Vadakkepat, AK and Redzej, A and Lukoyanova, N and Oomen, C and Braun, N and Ukleja, M and Lu, F and Dias, da Costa T and Orlova, EV and Baker, D and Cong, Q and Waksman, G},
doi = {10.1038/s41586-022-04859-y},
journal = {Nature},
title = {Cryo-EM structure of a type IV secretion system},
url = {http://dx.doi.org/10.1038/s41586-022-04859-y},
volume = {607},
year = {2022}
}

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TY  - JOUR
AB  - Bacterial conjugation is the fundamental process of unidirectional transfer of DNAs, often plasmid DNAs, from a donor cell to a recipient cell1. It is the primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-negative bacteria, conjugation is mediated by a large transport apparatus-the conjugative type IV secretion system (T4SS)-produced by the donor cell and embedded in both its outer and inner membranes. The T4SS also elaborates a long extracellular filament-the conjugative pilus-that is essential for DNA transfer4,5. Here we present a high-resolution cryo-electron microscopy (cryo-EM) structure of a 2.8 megadalton T4SS complex composed of 92 polypeptides representing 8 of the 10 essential T4SS components involved in pilus biogenesis. We added the two remaining components to the structural model using co-evolution analysis of protein interfaces, to enable the reconstitution of the entire system including the pilus. This structure describes the exceptionally large protein-protein interaction network required to assemble the many components that constitute a T4SS and provides insights on the unique mechanism by which they elaborate pili.
AU  - Macé,K
AU  - Vadakkepat,AK
AU  - Redzej,A
AU  - Lukoyanova,N
AU  - Oomen,C
AU  - Braun,N
AU  - Ukleja,M
AU  - Lu,F
AU  - Dias,da Costa T
AU  - Orlova,EV
AU  - Baker,D
AU  - Cong,Q
AU  - Waksman,G
DO  - 10.1038/s41586-022-04859-y
PY  - 2022///
SN  - 0028-0836
TI  - Cryo-EM structure of a type IV secretion system
T2  - Nature
UR  - http://dx.doi.org/10.1038/s41586-022-04859-y
UR  - https://www.ncbi.nlm.nih.gov/pubmed/35732732
UR  - https://www.nature.com/articles/s41586-022-04859-y
UR  - http://hdl.handle.net/10044/1/98034
VL  - 607
ER  - 

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