Location: Level 7 Sir Michael Uren Hub, White City Campus
Technique: DNA amplification
Manufacturer: Applied Biosystems
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Real-time PCR (qPCR) allows the specific detection and quantification of nucleic acid targets. Unlike traditional PCR amplification, qPCR includes the additional detection of a fluorescent signal after each cycle to monitor the number of DNA amplicons in the sample as the reaction progresses, eliminating the need for an extra gel electrophoresis detection step. This in turn allows the determination of the absolute quantity of target DNA in the original sample.

Real-time PCR assays have become the tool of choice for the rapid and sensitive determination and quantitation of nucleic acid in various biological samples, with diverse applications such as gene expression analysis.