Microscopic image of mitochondriaThis page contains a list of useful web links and documents relating to sample preparation, fluorophores (dyes) and other imaging requirements


General information

Spectral information on fluorophores, filters and lamps


  • Nyquist Calculator - calculates the optimal sampling rate for microscopic image acquisition (Nyquist rate = 2x maximal resolution) Scientific Volume Imaging

General imaging and analysis software

Tutorial: guidance for quantitative confocal microscopy by James Jonkman, Claire M. Brown, Graham D. Wright, Kurt I. Anderson and Alison J. North. Nature Protocols 2020

See our Software Pages - Software


Whole figure preparation

Protocols, tips and tricks - sample preparation

This is a growing collection of methods and techniques used in the facility, representing the enormous range of expertise of our microscopists. Everybody is very much invited to contribute with their own protocols. Protocols are provided as Word files, so they can be downloaded and edited as required.