In this section
@article{Adachi:2020:10.7554/eLife.49956,
author = {Adachi, H and Contreras, MP and Harant, A and Wu, C-H and Derevnina, L and Sakai, T and Duggan, C and Moratto, E and Bozkurt, TO and Maqbool, A and Win, J and Kamoun, S},
doi = {10.7554/eLife.49956},
journal = {eLife},
pages = {1--31},
title = {An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species},
url = {http://dx.doi.org/10.7554/eLife.49956},
volume = {8},
year = {2020}
}
TY - JOUR
AB - The molecular codes underpinning the functions of plant NLR immune receptors are poorly understood. We used in vitro Mu transposition to generate a random truncation library and identify the minimal functional region of NLRs. We applied this method to NRC4—a helper NLR that functions with multiple sensor NLRs within a Solanaceae receptor network. This revealed that the NRC4 N-terminal 29 amino acids are sufficient to induce hypersensitive cell death. This region is defined by the consensus MADAxVSFxVxKLxxLLxxEx (MADA motif) that is conserved at the N-termini of NRC family proteins and ~20% of coiled-coil (CC)-type plant NLRs. The MADA motif matches the N-terminal α1 helix of Arabidopsis NLR protein ZAR1, which undergoes a conformational switch during resistosome activation. Immunoassays revealed that the MADA motif is functionally conserved across NLRs from distantly related plant species. NRC-dependent sensor NLRs lack MADA sequences indicating that this motif has degenerated in sensor NLRs over evolutionary time.
AU - Adachi,H
AU - Contreras,MP
AU - Harant,A
AU - Wu,C-H
AU - Derevnina,L
AU - Sakai,T
AU - Duggan,C
AU - Moratto,E
AU - Bozkurt,TO
AU - Maqbool,A
AU - Win,J
AU - Kamoun,S
DO - 10.7554/eLife.49956
EP - 31
PY - 2020///
SN - 2050-084X
SP - 1
TI - An N-terminal motif in NLR immune receptors is functionally conserved across distantly related plant species
T2 - eLife
UR - http://dx.doi.org/10.7554/eLife.49956
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000505944500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://elifesciences.org/articles/49956
VL - 8
ER -
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