BibTex format
@article{Lee:2008:10.1111/j.1462-5822.2007.01065.x,
author = {Lee, SF and Kelly, M and McAlister, A and Luck, SN and Garcia, EL and Hall, RA and Robins-Browne, RM and Frankel, G and Hartland, EL},
doi = {10.1111/j.1462-5822.2007.01065.x},
journal = {CELL MICROBIOL},
pages = {499--513},
title = {A C-terminal class IPDZ binding motif of EspI/NIeA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium},
url = {http://dx.doi.org/10.1111/j.1462-5822.2007.01065.x},
volume = {10},
year = {2008}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Enteropathogenic Escherichia coli induces characteristic attaching-effacing (A/E) lesions on the intestinal mucosa during infection. The locus of enterocyte effacement is essential for A/E lesion formation and encodes a type III secretion system that translocates multiple effector proteins into the host cell. Following translocation, EspI/NIeA localizes to the Golgi. Using the yeast two-hybrid system (Y2HS) and PSD-95/Disk-large/ZO-1 (PDZ)-domain protein array overlays, we identified 15 putative host-interacting partners of Espl. All but two of the target proteins contained PDZ domains. Examination of the EspI amino acid sequence revealed a C-terminal consensus class I PDZ binding motif. Deletion of the last 7 amino acids of EspI to generate EspI(Delta C7) abrogated the Y2HS interaction between EspI and 5 of the 6 putative host cell target proteins tested. Deletion of the EspI PDZ binding motif also resulted in delayed trafficking of EspI to the Golgi. Using a mouse model of infection, we showed that Citrobacter rodentium expressing truncated EspI(Delta C7) was attenuated when in competition with C. rodentium expressing full-length EspI. Overall, these results suggested that Espl may modulate the virulence of A/E pathogens by binding host PDZ-domain proteins.
AU - Lee,SF
AU - Kelly,M
AU - McAlister,A
AU - Luck,SN
AU - Garcia,EL
AU - Hall,RA
AU - Robins-Browne,RM
AU - Frankel,G
AU - Hartland,EL
DO - 10.1111/j.1462-5822.2007.01065.x
EP - 513
PY - 2008///
SN - 1462-5814
SP - 499
TI - A C-terminal class IPDZ binding motif of EspI/NIeA modulates the virulence of attaching and effacing Escherichia coli and Citrobacter rodentium
T2 - CELL MICROBIOL
UR - http://dx.doi.org/10.1111/j.1462-5822.2007.01065.x
VL - 10
ER -