In this section
@article{Eulalio:2007:10.1101/gad.443107,
author = {Eulalio, A and Rehwinkel, J and Stricker, M and Huntzinger, E and Yang, SF and Doerks, T and Dorner, S and Bork, P and Boutros, M and Izaurralde, E},
doi = {10.1101/gad.443107},
journal = {Genes Dev},
pages = {2558--2570},
title = {Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing},
url = {http://dx.doi.org/10.1101/gad.443107},
volume = {21},
year = {2007}
}
TY - JOUR
AB - microRNAs (miRNAs) silence gene expression by suppressing protein production and/or by promoting mRNA decay. To elucidate how silencing is accomplished, we screened an RNA interference library for suppressors of miRNA-mediated regulation in Drosophila melanogaster cells. In addition to proteins known to be required for miRNA biogenesis and function (i.e., Drosha, Pasha, Dicer-1, AGO1, and GW182), the screen identified the decapping activator Ge-1 as being required for silencing by miRNAs. Depleting Ge-1 alone and/or in combination with other decapping activators (e.g., DCP1, EDC3, HPat, or Me31B) suppresses silencing of several miRNA targets, indicating that miRNAs elicit mRNA decapping. A comparison of gene expression profiles in cells depleted of AGO1 or of individual decapping activators shows that approximately 15% of AGO1-targets are also regulated by Ge-1, DCP1, and HPat, whereas 5% are dependent on EDC3 and LSm1-7. These percentages are underestimated because decapping activators are partially redundant. Furthermore, in the absence of active translation, some miRNA targets are stabilized, whereas others continue to be degraded in a miRNA-dependent manner. These findings suggest that miRNAs mediate post-transcriptional gene silencing by more than one mechanism.
AU - Eulalio,A
AU - Rehwinkel,J
AU - Stricker,M
AU - Huntzinger,E
AU - Yang,SF
AU - Doerks,T
AU - Dorner,S
AU - Bork,P
AU - Boutros,M
AU - Izaurralde,E
DO - 10.1101/gad.443107
EP - 2570
PY - 2007///
SN - 0890-9369
SP - 2558
TI - Target-specific requirements for enhancers of decapping in miRNA-mediated gene silencing
T2 - Genes Dev
UR - http://dx.doi.org/10.1101/gad.443107
UR - https://www.ncbi.nlm.nih.gov/pubmed/17901217
VL - 21
ER -
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