In this section
@article{Eulalio:2007:10.1128/MCB.00128-07,
author = {Eulalio, A and Behm-Ansmant, I and Schweizer, D and Izaurralde, E},
doi = {10.1128/MCB.00128-07},
journal = {Mol Cell Biol},
pages = {3970--3981},
title = {P-body formation is a consequence, not the cause, of RNA-mediated gene silencing},
url = {http://dx.doi.org/10.1128/MCB.00128-07},
volume = {27},
year = {2007}
}
TY - JOUR
AB - P bodies are cytoplasmic domains that contain proteins involved in diverse posttranscriptional processes, such as mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing. The localization of these proteins and their targets in P bodies raises the question of whether their spatial concentration in discrete cytoplasmic domains is required for posttranscriptional gene regulation. We show that processes such as mRNA decay, NMD, and RNA-mediated gene silencing are functional in cells lacking detectable microscopic P bodies. Although P bodies are not required for silencing, blocking small interfering RNA or microRNA silencing pathways at any step prevents P-body formation, indicating that P bodies arise as a consequence of silencing. Consistently, we show that releasing mRNAs from polysomes is insufficient to trigger P-body assembly: polysome-free mRNAs must enter silencing and/or decapping pathways to nucleate P bodies. Thus, even though P-body components play crucial roles in mRNA silencing and decay, aggregation into P bodies is not required for function but is instead a consequence of their activity.
AU - Eulalio,A
AU - Behm-Ansmant,I
AU - Schweizer,D
AU - Izaurralde,E
DO - 10.1128/MCB.00128-07
EP - 3981
PY - 2007///
SN - 0270-7306
SP - 3970
TI - P-body formation is a consequence, not the cause, of RNA-mediated gene silencing
T2 - Mol Cell Biol
UR - http://dx.doi.org/10.1128/MCB.00128-07
UR - https://www.ncbi.nlm.nih.gov/pubmed/17403906
VL - 27
ER -
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