BibTex format
@article{Dejonghe:2016:10.1038/ncomms11710,
author = {Dejonghe, W and Kuenen, S and Mylle, E and Vasileva, M and Keech, O and Viotti, C and Swerts, J and Fendrych, M and Ortiz-Morea, FA and Mishev, K and Delang, S and Scholl, S and Zarza, X and Heilmann, M and Kourelis, J and Kasprowicz, J and Nguyen, LSL and Drozdzecki, A and Van, Houtte I and Szatmári, A-M and Majda, M and Baisa, G and Bednarek, SY and Robert, S and Audenaert, D and Testerink, C and Munnik, T and Van, Damme D and Heilmann, I and Schumacher, K and Winne, J and Friml, J and Verstreken, P and Russinova, E},
doi = {10.1038/ncomms11710},
journal = {Nature Communications},
title = {Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification},
url = {http://dx.doi.org/10.1038/ncomms11710},
volume = {7},
year = {2016}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - ATP production requires the establishment of an electrochemical proton gradient across the inner mitochondrial membrane. Mitochondrial uncouplers dissipate this proton gradient and disrupt numerous cellular processes, including vesicular trafficking, mainly through energy depletion. Here we show that Endosidin9 (ES9), a novel mitochondrial uncoupler, is a potent inhibitor of clathrin-mediated endocytosis (CME) in different systems and that ES9 induces inhibition of CME not because of its effect on cellular ATP, but rather due to its protonophore activity that leads to cytoplasm acidification. We show that the known tyrosine kinase inhibitor tyrphostinA23, which is routinely used to block CME, displays similar properties, thus questioning its use as a specific inhibitor of cargo recognition by the AP-2 adaptor complex via tyrosine motif-based endocytosis signals. Furthermore, we show that cytoplasm acidification dramatically affects the dynamics and recruitment of clathrin and associated adaptors, and leads to reduction of phosphatidylinositol 4,5-biphosphate from the plasma membrane.
AU - Dejonghe,W
AU - Kuenen,S
AU - Mylle,E
AU - Vasileva,M
AU - Keech,O
AU - Viotti,C
AU - Swerts,J
AU - Fendrych,M
AU - Ortiz-Morea,FA
AU - Mishev,K
AU - Delang,S
AU - Scholl,S
AU - Zarza,X
AU - Heilmann,M
AU - Kourelis,J
AU - Kasprowicz,J
AU - Nguyen,LSL
AU - Drozdzecki,A
AU - Van,Houtte I
AU - Szatmári,A-M
AU - Majda,M
AU - Baisa,G
AU - Bednarek,SY
AU - Robert,S
AU - Audenaert,D
AU - Testerink,C
AU - Munnik,T
AU - Van,Damme D
AU - Heilmann,I
AU - Schumacher,K
AU - Winne,J
AU - Friml,J
AU - Verstreken,P
AU - Russinova,E
DO - 10.1038/ncomms11710
PY - 2016///
SN - 2041-1723
TI - Mitochondrial uncouplers inhibit clathrin-mediated endocytosis largely through cytoplasmic acidification
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/ncomms11710
UR - https://www.ncbi.nlm.nih.gov/pubmed/27271794
UR - https://www.nature.com/articles/ncomms11710
VL - 7
ER -