In this section
@article{Ortega:2014:10.1021/nl500234t,
author = {Ortega, Arroyo J and Andrecka, J and Spillane, KM and Billington, N and Takagi, Y and Sellers, JR and Kukura, P},
doi = {10.1021/nl500234t},
journal = {Nano Lett},
pages = {2065--2070},
title = {Label-free, all-optical detection, imaging, and tracking of a single protein.},
url = {http://dx.doi.org/10.1021/nl500234t},
volume = {14},
year = {2014}
}
TY - JOUR
AB - Optical detection of individual proteins requires fluorescent labeling. Cavity and plasmonic methodologies enhance single molecule signatures in the absence of any labels but have struggled to demonstrate routine and quantitative single protein detection. Here, we used interferometric scattering microscopy not only to detect but also to image and nanometrically track the motion of single myosin 5a heavy meromyosin molecules without the use of labels or any nanoscopic amplification. Together with the simple experimental arrangement, an intrinsic independence from strong electronic transition dipoles and a detection limit of <60 kDa, our approach paves the way toward nonresonant, label-free sensing and imaging of nanoscopic objects down to the single protein level.
AU - Ortega,Arroyo J
AU - Andrecka,J
AU - Spillane,KM
AU - Billington,N
AU - Takagi,Y
AU - Sellers,JR
AU - Kukura,P
DO - 10.1021/nl500234t
EP - 2070
PY - 2014///
SP - 2065
TI - Label-free, all-optical detection, imaging, and tracking of a single protein.
T2 - Nano Lett
UR - http://dx.doi.org/10.1021/nl500234t
UR - https://www.ncbi.nlm.nih.gov/pubmed/24597479
VL - 14
ER -
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