@article{Tica:2024:10.1021/acssynbio.4c00068,
author = {Tica, J and Chen, H and LUO, S and Chen, M and Isalan, M},
doi = {10.1021/acssynbio.4c00068},
journal = {ACS Synthetic Biology},
title = {Engineering tunable, low latency spatial computation with dual input quorum sensing promoters},
url = {http://dx.doi.org/10.1021/acssynbio.4c00068},
year = {2024}
}

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TY  - JOUR
AB  - Quorum sensing signals have evolved for population-level signaling in bacterial communities and are versatile tools for engineering cell–cell signaling in synthetic biology projects. Here, we characterize the spatial diffusion of a palette of quorum sensing signals and find that their diffusion in agar can be predicted from their molecular weight with a simple power law. We also engineer novel dual- and multi-input promoters that respond to quorum-sensing diffusive signals for use in engineered genetic systems. We engineer a promoter scaffold that can be adapted for activation and repression by multiple diffusers simultaneously. Lastly, we combine the knowledge on diffusion dynamics with the novel genetic components to build a new generation of spatial, stripe-forming systems with a simplified design, improved robustness, tuneability, and response time.
AU  - Tica,J
AU  - Chen,H
AU  - LUO,S
AU  - Chen,M
AU  - Isalan,M
DO  - 10.1021/acssynbio.4c00068
PY  - 2024///
SN  - 2161-5063
TI  - Engineering tunable, low latency spatial computation with dual input quorum sensing promoters
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/acssynbio.4c00068
UR  - https://pubs.acs.org/doi/10.1021/acssynbio.4c00068
UR  - http://hdl.handle.net/10044/1/111885
ER  - 

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