BibTex format
@article{Aruta:2022:10.3390/mps5060100,
author = {Aruta, MG and De, Simone D and Dale, H and Chirwa, E and Kadwala, I and Mbewe, M and Banda, H and Gordon, M and Pizza, M and Berlanda, Scorza F and Nyirenda, T and Canals, R and Rossi, O},
doi = {10.3390/mps5060100},
journal = {Methods and Protocols},
pages = {100--100},
title = {Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bactericidal Activity of Human Sera against Nontyphoidal Salmonella},
url = {http://dx.doi.org/10.3390/mps5060100},
volume = {5},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - <jats:p>Salmonella Typhimurium and Salmonella Enteritidis are leading causative agents of invasive nontyphoidal Salmonella (iNTS) disease, which represents one of the major causes of death and morbidity in sub-Saharan Africa, still partially underestimated. Large sero-epidemiological studies are necessary to unravel the burden of disease and guide the introduction of vaccines that are not yet available. Even if no correlate of protection has been determined so far for iNTS, the evaluation of complement-mediated functionality of antibodies generated towards natural infection or elicited upon vaccination may represent a big step towards this achievement. Here we present the setup and the intra-laboratory characterization in terms of repeatability, intermediate precision, linearity, and specificity of a high-throughput luminescence-based serum bactericidal assay (L-SBA). This method could be useful to perform sero-epidemiological studies across iNTS endemic countries and for evaluation of antibodies raised against iNTS vaccine candidates in upcoming clinical trials.</jats:p>
AU - Aruta,MG
AU - De,Simone D
AU - Dale,H
AU - Chirwa,E
AU - Kadwala,I
AU - Mbewe,M
AU - Banda,H
AU - Gordon,M
AU - Pizza,M
AU - Berlanda,Scorza F
AU - Nyirenda,T
AU - Canals,R
AU - Rossi,O
DO - 10.3390/mps5060100
EP - 100
PY - 2022///
SP - 100
TI - Development and Characterization of a Luminescence-Based High-Throughput Serum Bactericidal Assay (L-SBA) to Assess Bactericidal Activity of Human Sera against Nontyphoidal Salmonella
T2 - Methods and Protocols
UR - http://dx.doi.org/10.3390/mps5060100
VL - 5
ER -