BibTex format
@article{Cheong:2025:10.1128/spectrum.00356-25,
author = {Cheong, B and He, C and Laurenson, I and Claxton, P and Kostrzewa, M and Drobniewski, F and Larrouy-Maumus, G},
doi = {10.1128/spectrum.00356-25},
journal = {Microbiology Spectrum},
title = {Performance of direct detection of Mycobacterium tuberculosis within Mycobacterium tuberculosis complex by routine MALDI-ToF for the diagnosis using species-specific lipid fingerprint},
url = {http://dx.doi.org/10.1128/spectrum.00356-25},
volume = {13},
year = {2025}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Managing tuberculosis cases requires species and drug susceptibility identification, which are limited by the time taken by testing procedures due to slow bacterial growth. Lipid-based matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for identifying pathogenic mycobacterial species. This study aims to define and use species-specific lipid profiles of members of the Mycobacterium tuberculosis complex (MTBC) obtained by MALDI-TOF MS, to directly discriminate M. tuberculosis from other members of the MTBC, such as Mycobacterium africanum, Mycobacterium bovis, and M. bovis bacillus Calmette-Guerin (BCG). Reference strains (M. tuberculosis H37Rv, M. africanum, M. bovis, and M. bovis BCG) were grown in Middlebrook 7H11 media (supplemented with 10% oleic acid-albumin-dextrose-catalase growth supplement) and incubated for up to 6 weeks at 37°C to generate the large biomass (~109 bacteria) required for optimization, to assess reproducibility of the assay, and to set the reference lipid database. In clinical use, standard shorter culture periods would be sufficient. A blinded study was then performed using a collection of 46 mycobacterial clinical isolate strains composed of 30 M. tuberculosis, 2 M. africanum, 9 M. bovis BCG, and 5 M. bovis and grown under the same conditions. Cultured mycobacteria were heat-inactivated and loaded onto the matrix-assisted laser desorption target, followed by the addition of the matrix. Acquisition of the data was done using the negative ion mode. Using the species-specific glycolipid, sulfolipids, M. tuberculosis was discriminated within MTBC using the MALDI-TOF process with a sensitivity and specificity of 86.7% (95% confidence interval [CI] 69.3–96.2) and 93.7% (95% CI 69.8–99.8), respectively. Direct detection of M. tuberculosis within the MTBC based on mycobacterial lipid profiling provides a safe and accurate method, based on the detection of the sulfolipids
AU - Cheong,B
AU - He,C
AU - Laurenson,I
AU - Claxton,P
AU - Kostrzewa,M
AU - Drobniewski,F
AU - Larrouy-Maumus,G
DO - 10.1128/spectrum.00356-25
PY - 2025///
SN - 2165-0497
TI - Performance of direct detection of Mycobacterium tuberculosis within Mycobacterium tuberculosis complex by routine MALDI-ToF for the diagnosis using species-specific lipid fingerprint
T2 - Microbiology Spectrum
UR - http://dx.doi.org/10.1128/spectrum.00356-25
UR - https://journals.asm.org/doi/full/10.1128/spectrum.00356-25
VL - 13
ER -