In this section
@article{Cheong:2025,
author = {Cheong, B and Laurenson, I and Claxton, P and Kostrzewa, M and Drobniewski, F and Larrouy-Maumus, G},
journal = {Microbiology Spectrum},
title = {Performance of direct detection of Mycobacterium tuberculosis within Mycobacterium tuberculosis complex by routine MALDI-ToF for the diagnosis using species-specific lipid fingerprint},
year = {2025}
}
TY - JOUR
AB - Objectives:Managing tuberculosis cases require species and drug susceptibility identification, which are limited by the time taken by testing procedures due to slow bacterial growth. Lipid-based matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) is a promising tool for identifying pathogenic mycobacteria species. This study aims to define and use species-specific lipid profiles of members of the Mycobacterium tuberculosis complex (MTBC) obtained by MALDI-TOF MS, to directly discriminate M. tuberculosis from other members of the MTBC such as M. africanum, M. bovis, M. bovis BCG. Methods:Reference strains (M. tuberculosis H37Rv, M. africanum, M. bovis, M. bovis BCG) were grown in Middlebrook 7H11 media (supplemented with 10% OADC growth supplement) and incubated for up to six weeks at 37°C, to generate large biomass (~109 bacteria) required for optimization and assess reproducibility of the assay and used to set the reference lipid database. In clinical use standard shorter culture periods would be sufficient.A blinded study was then performed using a collection of 46 mycobacterial clinical isolates strains composed of 30 M. tuberculosis, 2 M. africanum, 9 M. bovis BCG, and 5 M. bovis and grown under the same conditions. Cultured mycobacteria were heat-inactivated and loaded onto the MALDI target followed by addition of the matrix. Acquisition of the data was done using the negative ion mode. Results:Using the species-specific glycolipid, sulfolipids, , M. tuberculosis was discriminated within MTBC using the MALDI-ToF process with a sensitivity and specificity of 86.7% (95% CI 69.3- 96.2) and 93.7% (95% CI 69.8- 99.8), respectively. Conclusions :Direct detection of M. tuberculosis within the MTB complex based on mycobacterial lipid profiling provides asafe and accurate method, based on the detection of the sulfolipids, as a species-specific lipid biomarker of M. tuberculosis.
AU - Cheong,B
AU - Laurenson,I
AU - Claxton,P
AU - Kostrzewa,M
AU - Drobniewski,F
AU - Larrouy-Maumus,G
PY - 2025///
SN - 2165-0497
TI - Performance of direct detection of Mycobacterium tuberculosis within Mycobacterium tuberculosis complex by routine MALDI-ToF for the diagnosis using species-specific lipid fingerprint
T2 - Microbiology Spectrum
ER -
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