BibTex format
@article{Habtewold:2025:10.1038/s41586-025-09685-6,
author = {Habtewold, T and Lwetoijera, DW and Hoermann, A and Mashauri, R and Matwewe, F and Mwanga, R and Kweyamba, P and Maganga, G and Magani, BP and Mtama, R and Mahonje, MA and Tambwe, MM and Tarimo, F and Chennuri, PR and Cai, JA and Del, Corsano G and Capriotti, P and Sasse, P and Moore, J and Hudson, D and Manjurano, A and Tarimo, B and Vlachou, D and Moore, S and Windbichler, N and Christophides, GK},
doi = {10.1038/s41586-025-09685-6},
journal = {Nature},
title = {Gene-drive-capable mosquitoes suppress patient-derived malaria in Tanzania.},
url = {http://dx.doi.org/10.1038/s41586-025-09685-6},
year = {2025}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Gene drive technology presents a transformative approach to combatting malaria by introducing genetic modifications into wild mosquito populations to reduce their vectorial capacity. Although effective modifications have been developed, these efforts have been confined to laboratories in the global north. We previously demonstrated that modifying Anopheles gambiae to express two exogenous antimicrobial peptides inhibits the sporogonic development of laboratory-cultured Plasmodium falciparum, with models predicting substantial contributions to malaria elimination in Africa when integrated with gene drive1-3. However, the effectiveness of this modification against genetically diverse, naturally circulating parasite isolates remained unknown. To address this critical gap, we adapted our technology for an African context by establishing infrastructural and research capacity in Tanzania, enabling the engineering of local A. gambiae under containment. Here we report the generation of a transgenic strain equipped with non-autonomous gene drive capabilities that robustly inhibits genetically diverse P. falciparum isolates obtained from naturally infected children. These genetic modifications were efficiently inherited by progeny when supplemented with Cas9 endonuclease provided by another locally engineered strain. Our work brings gene drive technology a critical step closer to application, providing a locally tailored and powerful tool for malaria eradication through the targeted dissemination of beneficial genetic traits in wild mosquito populations.
AU - Habtewold,T
AU - Lwetoijera,DW
AU - Hoermann,A
AU - Mashauri,R
AU - Matwewe,F
AU - Mwanga,R
AU - Kweyamba,P
AU - Maganga,G
AU - Magani,BP
AU - Mtama,R
AU - Mahonje,MA
AU - Tambwe,MM
AU - Tarimo,F
AU - Chennuri,PR
AU - Cai,JA
AU - Del,Corsano G
AU - Capriotti,P
AU - Sasse,P
AU - Moore,J
AU - Hudson,D
AU - Manjurano,A
AU - Tarimo,B
AU - Vlachou,D
AU - Moore,S
AU - Windbichler,N
AU - Christophides,GK
DO - 10.1038/s41586-025-09685-6
PY - 2025///
TI - Gene-drive-capable mosquitoes suppress patient-derived malaria in Tanzania.
T2 - Nature
UR - http://dx.doi.org/10.1038/s41586-025-09685-6
UR - https://www.ncbi.nlm.nih.gov/pubmed/41372414
ER -