In this section
@article{BRADY:1993:10.1002/j.1460-2075.1993.tb06186.x,
author = {BRADY, HJM and PENNINGTON, DJ and MILES, CG and DZIERZAK, EA},
doi = {10.1002/j.1460-2075.1993.tb06186.x},
journal = {The EMBO Journal},
pages = {4923--4932},
title = {CD4 cell surface downregulation in HIV1 Nef transgenic mice is a consequence of intracellular sequestration.},
url = {http://dx.doi.org/10.1002/j.1460-2075.1993.tb06186.x},
volume = {12},
year = {1993}
}
TY - JOUR
AB - The Nef gene product is a regulatory protein of HIV whose biological function is poorly understood. Nef has been thought to have a negative effect on viral replication in vitro but has been shown in studies with SIV to be necessary in the establishment of viraemia in vivo. In vitro studies in various human cell lines have shown that Nef downregulates the expression of cell surface CD4 and thus could have effects on the immune response. We have generated four transgenic mouse lines, with constructs containing two different Nef alleles under the control of CD2 regulatory elements to examine the interaction of Nef with the host immune system in vivo. In adult transgenic mice we have found marked downregulation in the level of CD4 on the surface of double positive thymocytes and a decrease in the number of CD4+ T cells in the thymus. Functional analyses have revealed a decrease in the total activation of transgenic thymocytes by antiCD3 epsilon antibody. By specific intracellular staining of T cells in such mice we have found CD4 colocalizing with a Golgispecific marker. These results strongly suggest a Nef mediated effect on developing CD4 thymocytes resulting from interference of Nef in the intracellular trafficking or posttranslational modification of CD4.
AU - BRADY,HJM
AU - PENNINGTON,DJ
AU - MILES,CG
AU - DZIERZAK,EA
DO - 10.1002/j.1460-2075.1993.tb06186.x
EP - 4932
PY - 1993///
SN - 0261-4189
SP - 4923
TI - CD4 cell surface downregulation in HIV1 Nef transgenic mice is a consequence of intracellular sequestration.
T2 - The EMBO Journal
UR - http://dx.doi.org/10.1002/j.1460-2075.1993.tb06186.x
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1993MM12000003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.embopress.org/doi/abs/10.1002/j.1460-2075.1993.tb06186.x
VL - 12
ER -
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