In this section
@article{Wiesler:2012:nar/gks844,
author = {Wiesler, SC and Burrows, PC and Buck, M},
doi = {nar/gks844},
journal = {Nucleic Acids Res},
pages = {10878--10892},
title = {A dual switch controls bacterial enhancer-dependent transcription},
url = {http://dx.doi.org/10.1093/nar/gks844},
volume = {40},
year = {2012}
}
TY - JOUR
AB - Bacterial RNA polymerases (RNAPs) are targets for antibiotics. Myxopyronin binds to the RNAP switch regions to block structural rearrangements needed for formation of open promoter complexes. Bacterial RNAPs containing the major variant sigma(54) factor are activated by enhancer-binding proteins (bEBPs) and transcribe genes whose products are needed in pathogenicity and stress responses. We show that (i) enhancer-dependent RNAPs help Escherichia coli to survive in the presence of myxopyronin, (ii) enhancer-dependent RNAPs partially resist inhibition by myxopyronin and (iii) ATP hydrolysis catalysed by bEBPs is obligatory for functional interaction of the RNAP switch regions with the transcription start site. We demonstrate that enhancer-dependent promoters contain two barriers to full DNA opening, allowing tight regulation of transcription initiation. bEBPs engage in a dual switch to (i) allow propagation of nucleated DNA melting from an upstream DNA fork junction and (ii) complete the formation of the transcription bubble and downstream DNA fork junction at the RNA synthesis start site, resulting in switch region-dependent RNAP clamp closure and open promoter complex formation.
AU - Wiesler,SC
AU - Burrows,PC
AU - Buck,M
DO - nar/gks844
EP - 10892
PY - 2012///
SN - 1362-4962
SP - 10878
TI - A dual switch controls bacterial enhancer-dependent transcription
T2 - Nucleic Acids Res
UR - http://dx.doi.org/10.1093/nar/gks844
UR - http://www.ncbi.nlm.nih.gov/pubmed/22965125
VL - 40
ER -
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